FEATURES OF STRUCTURE OF REPLICATING AND NON-REPLICATING CHROMATIN IN CHICKEN ERYTHROBLASTS
FEATURES OF STRUCTURE OF REPLICATING AND NON-REPLICATING CHROMATIN IN CHICKEN ERYTHROBLASTS
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DOI：
10.1093/nar/4.6.1881
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发表时间：
1977-01-01
影响因子：
14.9
通讯作者：
HEWISH, D
中科院分区：
文献类型：
--
作者：
HEWISH, D
在分离的细胞核中检测了DNA酶I对分离的鸡成红细胞合成的DNA的消化。发现新合成的DNA对DNA酶I敏感，但随着复制后时间的增加而成熟为相对抗性形式，如在哺乳动物系统中观察到的11，12。消化中胰蛋白酶的存在使所有DNA暴露于DNA酶I作用。对消化产物的检查表明，新复制的DNA在DNA-蛋白质复合物的结构上与更成熟的形式差别不大，但易感性的差异可能是DNA酶对新的和旧的DNA的访问速率不同的结果。
The digestion by DNAase I of DNA synthesised by isolated chicken erythroblasts was examined in isolated nuclei. It was found that newly synthesised DNA was susceptible to DNAase I but matured to a relatively resistant form with increasing time after replication as observed in mammalian systemsll,12. The presence of trypsin in the digestion exposed all of the DNA to DNAase I action. Examination of the digestion products showed that the newly replicated DNA differed little from the more mature form in the structure of the DNA-protein complex but that the difference in susceptibility was probably a result of a differential rate of access of the DNAase to the new and old DNA.