Comparison of the copy numbers of bovine leukemia virus in the lymph nodes of cattle with enzootic bovine leukosis and cattle with latent infection
Comparison of the copy numbers of bovine leukemia virus in the lymph nodes of cattle with enzootic bovine leukosis and cattle with latent infection
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DOI：
10.1007/s00705-014-2137-9
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发表时间：
2014-10-01
影响因子：
2.7
通讯作者：
Murakami, Kenji
中科院分区：
文献类型：
--
作者：
Somura, Yoshiko;Sugiyama, Emi;Murakami, Kenji
为了建立预测牛地方性白血病（EBL）的诊断指标，通过实时定量PCR（qPCR）检测全血、淋巴结和脾脏中的原病毒牛白血病病毒（BLV）拷贝。根据肉类检验数据，牛被分为两组：感染 EBL 的牛和感染 BLV 的牛。 EBL牛所有标本中BLV拷贝数均显着高于BLV感染牛（p < 0.0001），且淋巴结中BLV拷贝数特别多。 EBL 牛的颈浅、髂内和空肠淋巴结中超过 70% 的 DNA 含量超过 1,000 个拷贝/10 ng，而 BLV 感染牛的淋巴结则没有。这些发现表明，携带超过 1,000 个 BLV 拷贝的牛可能被诊断为 EBL。
To establish a diagnostic index for predicting enzootic bovine leukosis (EBL), proviral bovine leukemia virus (BLV) copies in whole blood, lymph nodes and spleen were examined by quantitative real-time PCR (qPCR). Cattle were divided into two groups, EBL and BLV-infected, based on meat inspection data. The number of BLV copies in all specimens of EBL cattle was significantly higher than those of BLV-infected cattle (p < 0.0001), and the number of BLV copies in the lymph nodes was particularly large. Over 70 % of the superficial cervical, medial iliac and jejunal lymph nodes from EBL cattle had more than 1,000 copies/10 ng DNA, whereas lymph nodes from BLV-infected cattle did not. These findings suggest that the cattle harboring more than 1,000 BLV copies may be diagnosed with EBL.