Intratracheal application of recombinant surfactant protein-C surfactant to rabbits attenuates acute lung injury induced by intratracheal acidified infant formula
Intratracheal application of recombinant surfactant protein-C surfactant to rabbits attenuates acute lung injury induced by intratracheal acidified infant formula
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DOI：
10.1213/01.ane.0000111111.76779.b3
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发表时间：
2004-05-01
影响因子：
5.7
通讯作者：
Obara, H
中科院分区：
文献类型：
--
作者：
Mikawa, K;Nishina, K;Obara, H
本研究的目的是确定重组表面活性蛋白-C(RSP-C)表面活性物质能否改善气管内酸化奶制品所致的急性肺损伤(ALI)。将28只兔随机分为4组。3组气管内注入酸化婴幼儿配方奶粉(0.8mL/kg，pH 1.8)诱导ALI。对照组气管内注入酸化生理盐水。治疗组在酸化婴儿配方奶粉后30min气管内注射RSP-C表面活性物质(0.5 mg/kg或2SP-C mg/kg)中的1剂。ALI诱导后，肺组织给予100%氧气4h。酸化的婴儿配方奶粉极大地降低了氧合能力和肺顺应性，增加了抵抗力。两种剂量的RSP-C均可改善变量[4h的平均动脉血氧分压(2)(毫米汞)和顺应性(毫升/厘米H2O)：婴儿配方奶粉分别为61和0.4，小剂量RSP-C分别为162和1.0，大剂量RSP-C分别为152和1.2；P<；0.05]。RSP-C治疗可减轻肺白细胞减少和水肿，以及严重的形态学改变(ALI评分：婴儿配方奶粉、小剂量RSP-C和大剂量RSP-C分别为14、7、7；P<；0.05)。两种剂量的RSP-C的疗效相似。这些结果表明，气管内给予RSP-C可改善吸入酸奶产品所致的ALI。
Our aim in the current study was to determine whether recombinant surfactant protein-C (rSP-C) surfactant improves acute lung injury (ALI) induced by intratracheal acidified milk products. Twenty-eight rabbits were randomly divided into four groups. ALI was induced with intratracheal acidified infant formula (0.8 mL/kg, pH 1.8) in 3 groups. The control group received intratracheal acidified saline. Therapy groups received 1 of 2 doses of intratracheal rSP-C surfactant (0.5 or 2 SP-C mg/kg) 30 min after the acidified infant formula. The lungs were ventilated with 100% oxygen for 4 h after induction of ALI. Acidified infant formula dramatically reduced oxygenation and lung compliance, and increased resistance. Both doses of rSP-C improved the variables [mean Pao(2) (mmHg) and compliance (mL/cm H2O) at 4 h: 61 and 0.4 for infant formula, 162 and 1.0 for small-dose rSP-C, and 152 and 1.2 for large-dose rSP-C, respectively; P < 0.05]. Pulmonary leukosequestration and edema, and severe morphological changes were attenuated by rSP-C treatment (ALI score: 14, 7, 7 in infant formula, small-dose rSP-C, and large-dose rSP-C; P < 0.05). The efficacy was similar for the two doses of rSP-C. These findings suggest that intratracheal administration of rSP-C ameliorates ALI induced by aspiration of acidified milk products.