Universally conserved translation initiation factors
Universally conserved translation initiation factors
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DOI：
10.1073/pnas.95.1.224
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发表时间：
1998-01-06
影响因子：
11.1
通讯作者：
Woese, CR
中科院分区：
文献类型：
--
作者：
Kyrpides, NC;Woese, CR
在细菌和真核生物中，翻译的启动过程一直被认为是相似的，但在这两种情况下，翻译是由一组不同的不相关因素完成的。这不仅表明两者的进化史不同，而且还表明在共同祖先阶段，翻译启动机制要么不存在，要么与现有过程具有不同的性质。我们在这里证明(i)“类似”的翻译启动因子IF-1和eIF-1A实际上在序列上是相关的，（ii）“真核”翻译因子SUI1在分布上是普遍的。真核/古细菌翻译因子eIF-5A与细菌翻译因子EF-P同源。因此，翻译起始的雏形似乎已经出现在普遍祖先阶段。然而，重要的发展和完善随后独立发生在细菌谱系和古细菌/真核细胞系。
The process by which translation is initiated has long been considered similar in Bacteria and Eukarya but accomplished by a different unrelated set of factors in the two cases. This not only implies separate evolutionary histories for the two but also implies that at the universal ancestor stage, a translation initiation mechanism either did not exist or was of a different nature than the extant processes, We demonstrate herein that (i) the "analogous" translation initiation factors IF-1 and eIF-1A are actually related in sequence, (ii) the "eukaryotic" translation factor SUI1 is universal in distribution, and (iii) the eukaryotic/archaeal translation factor eIF-5A is homologous to the bacterial translation factor EF-P. Thus, the rudiments of translation initiation would seem to have been present in the universal ancestor stage. However, significant development and refinement subsequently occurred independently on both the bacterial lineage and on the archaeal/eukaryotic line.