Quantification of glyphosate and aminomethylphosphonic acid from microbiome reactor fluids
Quantification of glyphosate and aminomethylphosphonic acid from microbiome reactor fluids
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DOI：
10.1002/rcm.8668
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发表时间：
2020-04-15
影响因子：
2
通讯作者：
Rolle-Kampczyk, Ulrike
中科院分区：
文献类型：
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作者：
Fritz-Wallace, Katarina;Engelmann, Beatrice;Rolle-Kampczyk, Ulrike
基本原理：草甘膦是最广泛使用的除草剂之一，它被怀疑通过抑制莽草酸途径合成芳香族氨基酸来影响肠道微生物群。体外微生物生物反应器越来越多地用作研究对肠道微生物群影响的模型系统，因此需要在微生物模型系统中定量草甘膦及其降解产物氨甲基膦酸（AMPA）的方法。一个优化的协议，使草甘膦和AMPA的分析，通过简单的提取与甲醇：乙腈：水（2：3：1），而无需进一步的富集步骤。采用液相色谱法在酰胺柱上分离甘氨酸和AMPA，并使用QTRAP 5500系统（AB Sciex）采用靶向串联质谱法进行鉴定和定量。
Rationale: Glyphosate is one of the most widely used herbicides and it is suspected to affect the intestinal microbiota through inhibition of aromatic amino acid synthesis via the shikimate pathway.In vitromicrobiome bioreactors are increasingly used as model systems to investigate effects on intestinal microbiota and consequently methods for the quantitation of glyphosate and its degradation product aminomethylphosphonic acid (AMPA) in microbiome model systems are required.Methods: An optimized protocol enables the analysis of both glyphosate and AMPA by simple extraction with methanol:acetonitrile:water (2:3:1) without further enrichment steps. Glyphosate and AMPA are separated by liquid chromatography on an amide column and identified and quantified with a targeted tandem mass spectrometry method using a QTRAP 5500 system (AB Sciex).Results: Our method has a limit of detection (LOD) in extracted water samples of