Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets.
Microscopy Image Browser: A Platform for Segmentation and Analysis of Multidimensional Datasets.
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DOI：
10.1371/journal.pbio.1002340
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发表时间：
2016-01
期刊：
影响因子：
9.8
通讯作者：
Jokitalo E
中科院分区：
文献类型：
--
作者：
Belevich I;Joensuu M;Kumar D;Vihinen H;Jokitalo E
要了解细胞和细胞器在其自然环境中的结构-功能关系，需要多维成像。随着多模式三维成像技术变得越来越容易获得，对大数据集的有效处理、可视化和分析正在成为工作流程的瓶颈。在这里，我们提出了一个用于多维数据集的高性能分割和图像处理的新软件包，该软件包改进并促进了对采集数据的充分利用和定量分析，该软件包可从专用网站免费获得。开放源码环境允许修改和插入新的插件，以根据特定需求定制程序。我们提供了用于处理、分割和分析光学和电子显微镜数据集的程序功能的实际示例，并提供了详细的教程，使用户能够快速而彻底地学习如何使用该程序。本社区网页描述了一个免费可用的开源软件，该软件实现并集成了一系列手动和半自动分割工具，用于处理和量化光学和电子显微镜数据。
Understanding the structure–function relationship of cells and organelles in their natural context requires multidimensional imaging. As techniques for multimodal 3-D imaging have become more accessible, effective processing, visualization, and analysis of large datasets are posing a bottleneck for the workflow. Here, we present a new software package for high-performance segmentation and image processing of multidimensional datasets that improves and facilitates the full utilization and quantitative analysis of acquired data, which is freely available from a dedicated website. The open-source environment enables modification and insertion of new plug-ins to customize the program for specific needs. We provide practical examples of program features used for processing, segmentation and analysis of light and electron microscopy datasets, and detailed tutorials to enable users to rapidly and thoroughly learn how to use the program. This Community Page describes a freely available, open-source software that implements and integrates a range of manual and semi-automated segmentation tools for processing and quantifying light and electron microscopy data.