Mycobacterium tuberculosis Glycoproteomics Based on ConA-Lectin Affinity Capture of Mannosylated Proteins
Mycobacterium tuberculosis Glycoproteomics Based on ConA-Lectin Affinity Capture of Mannosylated Proteins
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DOI：
10.1021/pr800756a
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发表时间：
2009-02-01
影响因子：
4.4
通讯作者：
Espitia, Clara
中科院分区：
文献类型：
--
作者：
Gonzalez-Zamnorano, Margarita;Mendoza-Hernandez, Guillermo;Espitia, Clara
结核分枝杆菌培养滤液富含含甘露糖蛋白的2-DE凝胶解析。ConA配体印迹后，41个蛋白质被鉴定为推定的糖蛋白，其中34个可能是新的甘露糖基化蛋白。这些结果有助于建立M.结核病，并提供有用的信息，了解糖蛋白在分枝杆菌的生物学作用。
A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.