CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin.
CdSe/ZnS quantum dots based electrochemical immunoassay for the detection of phosphorylated bovine serum albumin.
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DOI：
10.1016/j.bios.2010.08.021
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发表时间：
2010-11
影响因子：
12.6
通讯作者：
Kulwadee Pinwattana;Jun Wang;Chiann-Tso Lin;Hong Wu;Dan Du;Yuehe Lin;O. Chailapakul
中科院分区：
文献类型：
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作者：
Kulwadee Pinwattana;Jun Wang;Chiann-Tso Lin;Hong Wu;Dan Du;Yuehe Lin;O. Chailapakul
本文提出了一种基于CdSe/ZnS量子点的磷酸化牛血清白蛋白（BSA OP）电化学免疫分析方法。量子点被用作放大电化学信号的标记物，并在异相夹心免疫测定中与抗磷酸丝氨酸二抗缀合。在该试验中，将模型磷酸化蛋白BSA-OP加入到BSA一抗包被的聚苯乙烯微孔中，然后加入QD标记的抗磷酸丝氨酸抗体以完成免疫识别。最后，结合的QD在酸溶解步骤中溶解，并通过电化学溶出分析检测。在最佳条件下，BSA-OP在0.5- 500 ngmL − 1范围内呈线性关系，检出限为0.5ngmL−1。方法的相对标准偏差为8.6%，重现性好。这种基于量子点的电化学免疫分析为蛋白质生物标志物的简单和具有成本效益的分析提供了很大的希望。
A CdSe/ZnS quantum dot (QD) based electrochemical immunoassay of phosphorylated bovine serum albumin (BSA-OP) as a protein biomarker is presented. The QDs were used as labels for amplifying electrochemical signals and were conjugated with a secondary anti-phosphoserine antibody in a heterogeneous sandwich immunoassay. In this assay, the model phosphorylated protein BSA-OP was added to the primary BSA antibody coated polystyrene microwells, and then the QD labeled anti-phosphoserine antibody was added for completing immunorecognition. Finally, the bound QD was dissolved in an acid-dissolution step and was detected by electrochemical stripping analysis. The measured current responses were proportional to the concentration of BSA-OP. Under optimal conditions, the voltammetric response was linear over the range of 0.5–500ngmL−1of BSA-OP, with a detection limit of 0.5ngmL−1. It also shows good reproducibility with a relative standard deviation of 8.6%. This QD-based electrochemical immunoassay offers great promise for simple and cost-effective analysis of protein biomarkers.