Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics
Biological rhythmicity in expressed proteins of the marine dinoflagellate Lingulodinium polyedrum demonstrated by chronological proteomics
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DOI：
10.1016/j.bbrc.2004.01.054
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发表时间：
2004-03-05
影响因子：
3.1
通讯作者：
Ohmiya, Y
中科院分区：
文献类型：
--
作者：
Akimoto, H;Wu, C;Ohmiya, Y
甲藻显示出蛋白质的昼夜节律性表达。对大约900个蛋白质(等电点范围5-8；相对分子质量22,000-120,000)在12：12小时暗光(‘昼夜’)周期中的表达谱进行时间蛋白质组学分析，发现28个蛋白质具有定量的昼夜变化。这种模式被分为三种类型：阶段1(晚间)、阶段2(夜间)和阶段3(午夜)。多肽质量指纹图谱显示，荧光素结合蛋白存在于第二相，三羧酸循环的两个组分存在于第一相和第二相。因此，节律性似乎与生理状态有关，并控制着多年生乳杆菌的代谢途径。(C)2004 Elsevier Inc.保留所有权利。
The dinoflagellate Lingulodinium polyedrum shows circadian expression of proteins. Analysis of the expression profile of ca. 900 proteins (pI range 5-8; MW 22,000-120,000) by chronological proteornics in 12:12 h light dark ('day-night') cycles revealed 28 with quantitative circadian changes. The patterns were classified into three types: Phase 1 ('evening'), Phase 2 ('night'), and Phase 3 ('midnight'). Peptide mass fingerprinting identified luciferin-binding protein in Phase 2 and two components of the tricarboxylic acid cycle in Phases 1 and 2. Thus, rhythmicity appears to be related to physiological states and to control the metabolic pathway in L. polyedrum. (C) 2004 Elsevier Inc. All rights reserved.