Introduction of single-copy sequences into the chromosome of Escherichia coli: application to gene and operon fusions.
Introduction of single-copy sequences into the chromosome of Escherichia coli: application to gene and operon fusions.
复制标题
将单拷贝序列引入大肠杆菌染色体：应用于基因和操纵子融合。
DOI：
10.1016/0378-1119(91)90292-j
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
LaPorte,DC
中科院分区：
文献类型：
--
作者：
Resnik,E;LaPorte,DC
We have developed a general method for the introduction of any cloned sequence into the chromosome ofEscherichia coli. This method employs an Hfr strain which carries a fragment ofbla(the pBR322 gene imparting ampicillin resistance) betweenlacIandlacZ. Plasmid-borne inserts which are flanked by sequences fromblaandlacZcan be introduced at this locus by homologous recombination. The isolation of recombinants is enhanced by selection for transfer of an integrated copy of the plasmid during conjugation. Once introduced into the chromosome, the inserted sequences can be transferred to other strains by conventional methods such as P1 transduction or conjugation. This method is suitable for the transfer of any cloned sequence to the chromosome and is particularly well suited to the construction of chromosomal gene and operon fusions withlacZ.
