Selective deposits of versican in the extracellular matrix of restenotic lesions from human peripheral arteries.
Selective deposits of versican in the extracellular matrix of restenotic lesions from human peripheral arteries.
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发表时间：
1997-10
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通讯作者：
T. Wight;S. Lara;Reimer Riessen;R. Le;Baron;J. Isner
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作者：
T. Wight;S. Lara;Reimer Riessen;R. Le;Baron;J. Isner
虽然很大比例的人再狭窄动脉病变的体积被细胞外基质（ECM）占据，但这种ECM的组成和组织还没有得到很好的表征。在这项研究中，从30个人外周动脉血管成形术后的时间范围从13天到36个月的定向粥样斑块切除术的再狭窄段进行了分析的ECM组成和组织的光和电子显微镜组织化学和免疫组化的特定模式。组织化学分析显示存在不同的区域，富含蛋白聚糖或纤维胶原。大多数部分包含这些相互并列的区域。血管成形术后，这两种不同ECM的频率并不随时间而变化。胶原蛋白丰富的区域通常含有细长的平滑肌细胞，除了类似纤维斑块的区域外，这些细胞间隔很近。富含蛋白多糖的ECM包含随机排列的长形和星状平滑肌细胞，并相隔很远。该区域类似于再狭窄病变典型的含疏松结缔组织的粘液样区域。这些地区的免疫组织化学分析表明，蛋白聚糖含有区染色强烈的多能蛋白聚糖，一个大的间质硫酸软骨素蛋白聚糖，而含胶原蛋白的地区大多是阴性的多能蛋白聚糖，但阳性的I型胶原蛋白。versican阳性区域也对双糖链蛋白聚糖（一种富含亮氨酸的硫酸皮肤素蛋白聚糖）进行免疫染色，并对弹性蛋白进行稀疏染色。然而，这两种ECM分子都存在于病变的versican阴性I型胶原阳性区域。这些结果表明，再狭窄病变的发展涉及特定ECM分子的局部沉积，这些ECM分子可能在血管成形术后该组织的不对称再狭窄中发挥作用。
Although a large percentage of the volume of human restenotic arterial lesions is occupied by extracellular matrix (ECM), the composition and organization of this ECM are not well characterized. In this study, restenotic segments taken from 30 human peripheral arteries by directional atherectomy at times ranging from 13 days to 36 months after angioplasty were analyzed for specific patterns of ECM composition and organization by light and electron microscopic histochemistry and immunohistochemistry. Histochemical analysis revealed the presence of distinct zones, enriched either in proteoglycans or fibrillar collagen. Most sections contained these regions juxtaposed to each other. The frequency of these two distinct ECMs did not change as a function of time after angioplasty. The collagen-rich zone usually contained elongated smooth muscle cells spaced close together except in regions resembling fibrous plaques. The proteoglycan-rich ECM contained both elongated and stellate-shaped smooth muscle cells randomly arranged and separated by wide distances. This region resembled the loose-connective-tissue-containing myxoid region typical of restenotic lesions. Immunohistochemical analysis of these regions revealed that the proteoglycan-containing zone stained intensely for versican, a large interstitial chondroitin sulfate proteoglycan, whereas the collagen-containing areas were mostly negative for versican but positive for type I collagen. The versican-positive regions also immunostained for biglycan, a small leucine-rich dermatan sulfate proteoglycan, and sparsely for elastin. However, both of these ECM molecules were present in the versican-negative type I collagen-positive regions of the lesions. These results suggest that the development of restenotic lesions involves localized deposits of specific ECM molecules that may play a role in the asymmetric renarrowing of this tissue after angioplasty.