Oleic acid-induced mucosal injury in developing piglet intestine.
Oleic acid-induced mucosal injury in developing piglet intestine.
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DOI：
10.1152/ajpgi.1993.264.3.g576
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发表时间：
1993-03
期刊：
影响因子：
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通讯作者：
O. R. Velásquez;K. Henninger;M. Fowler;P. Tso;K. Crissinger
中科院分区：
文献类型：
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作者：
O. R. Velásquez;K. Henninger;M. Fowler;P. Tso;K. Crissinger
管腔营养物质，特别是脂质消化产物，在发育中的肠粘膜损伤的发病机制中的作用已经被假定。我们评估了随着年龄和脂肪酸浓度的变化，在发展中的仔猪肠腔灌注与长链脂肪酸油酸酯诱导的粘膜渗透性和光镜和电镜组织学的变化。在依次灌注生理盐水对照（20 min）、0、1、5和10 mM油酸/10 mM牛磺胆酸盐生理盐水（20 min）和生理盐水（60 min）的过程中，测量了1日龄、3日龄、2周龄和1月龄仔猪空肠和回肠中51 Cr标记EDTA血浆-管腔清除率。与1月龄的动物相比，在用油酸灌注后，小于或等于2周的仔猪空肠的粘膜渗透性显示出显著更大的增加。这种效应依赖于脂肪酸的管腔浓度，并与光镜和电镜下明显的粘膜损伤有关。相比之下，回肠的总体反应比空肠更弱。因此，油酸，一种常见的膳食脂肪酸，诱导剂量和年龄依赖性的损伤，在发育中的仔猪肠道。这种损伤的机制的调查可能提供的基础上，针对饮食的调整，以减少在新生儿肠道喂养过程中的粘膜损伤的风险。
A role for luminal nutrients, in particular products of lipid digestion, in the pathogenesis of mucosal injury to developing intestine has been postulated. We evaluated changes in mucosal permeability and light and electron microscopic histology induced by luminal perfusion with the long-chain fatty acid oleate in developing piglet intestine as a function of age and concentration of the fatty acid. 51Cr-labeled EDTA plasma-to-lumen clearance was measured in jejunum and ileum of 1-day-, 3-day-, 2-wk-, and 1-mo-old piglets during sequential perfusion with saline control (20 min); 0, 1, 5, and 10 mM oleic acid/10 mM taurocholate in saline (20 min); and normal saline (60 min). The jejunum of piglets < or = 2 wk showed significantly greater increases in mucosal permeability compared with 1-mo-old animals after perfusion with oleic acid. This effect was dependent on the luminal concentration of the fatty acid and was associated with mucosal injury evident under light and electron microscopy. In contrast, the overall response in ileum was more attenuated compared with jejunum. Thus oleic acid, a common dietary fatty acid, induces dose- and age-dependent injury in developing piglet intestine. Investigation of the mechanisms of this injury may provide the basis for dietary modifications directed at decreasing the risk of mucosal injury during enteral feeding in neonatal intestine.