Role of antibody and complement in opsonization of group B streptococci
Role of antibody and complement in opsonization of group B streptococci
复制标题
抗体和补体在 B 族链球菌调理作用中的作用
作者：
A. Shigeoka;R. Hall;V. Hemming;C. Allred;H. Hill
用化学发光法和放射性标记细菌摄取技术观察到在B组链球菌的调理中需要经典的补体途径。经典途径增加了Ia型和II型股票和野生菌株以及一些III型野生菌株的调理水平。相反，其他III型野生株和III型股票株在存在完整的经典途径的情况下有加速摄取的动力学，但调理水平与单独使用抗体时没有变化。我们不能证明替代途径在调理B群链球菌的原始株或野生株方面有显著作用。此外，溶血滴定的电泳和补体消耗分析未能揭示该组大多数菌株的替代途径激活。旨在为这些生物体提供调理素的治疗将需要存在特定类型的抗体。
A requirement for the classic complement pathway in opsonization of group B streptococci was observed by using both a chemiluminescence and a radiolabeled bacterial uptake technique. The classic pathway increased levels of opsonization for types Ia and II stock and wild strains and for some type III wild strains. In contrast, other type III wild strains and the type III stock strain had accelerated kinetics of uptake in the presence of an intact classic pathway, but the level of opsonization was unchanged from that with antibody alone. We could not demonstrate a significant role for the alternative pathway in opsonizing stock or wild strains of group B streptococci. Futhermore, electrophoretic and complement consumption analysis by hemolytic titration failed to reveal alternative pathway activation by the majority of strains of this group. Therapy aimed at supplying opsonins for these organisms will require the presence of type-specific antibody.