Natural variation of model mutant phenotypes in Ciona intestinalis.
Natural variation of model mutant phenotypes in Ciona intestinalis.
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DOI：
10.1371/journal.pone.0002344
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发表时间：
2008-06-04
期刊：
影响因子：
3.7
通讯作者：
Ristoratore F
中科院分区：
文献类型：
--
作者：
Sordino P;Andreakis N;Brown ER;Leccia NI;Squarzoni P;Tarallo R;Alfano C;Caputi L;D'Ambrosio P;Daniele P;D'Aniello E;D'Aniello S;Maiella S;Miraglia V;Russo MT;Sorrenti G;Branno M;Cariello L;Cirino P;Locascio A;Spagnuolo A;Zanetti L;Ristoratore F
海鞘类（脊索动物，被膜动物）的研究对我们理解基索动物的起源和演化做出了相当大的贡献。为了提供进一步的信息来支持玻璃海鞘的正向遗传学，我们使用了自然变异和中性群体遗传学的组合作为系统鉴定新突变的方法。除了发育变异对表型驱动研究的意义外，这种方法还可以在进化和种群生物学中产生重要影响。在这里，我们报告了一个初步的调查自然发生的突变，在三个地理上相互关联的人群的C。好吧利用12个微卫星分析了历史、地理和环境因素对异常表型分布的影响。我们确定了37个可能的突变基因座，在胚胎发育中的定型缺陷，以典型的隐性等位基因的方式分离。当地人口被发现不同的遗传组织和频率分布的表型类。自然遗传多态性C。海鞘是海鞘胚胎发育研究的重要表型来源。相关的遗传结构和异常表型的发生是一个至关重要的焦点，了解自然有限的人口形状的选择力，并可能提供见解的进化机制，产生动物的多样性非常重要。
The study of ascidians (Chordata, Tunicata) has made a considerable contribution to our understanding of the origin and evolution of basal chordates. To provide further information to support forward genetics in Ciona intestinalis, we used a combination of natural variation and neutral population genetics as an approach for the systematic identification of new mutations. In addition to the significance of developmental variation for phenotype-driven studies, this approach can encompass important implications in evolutionary and population biology. Here, we report a preliminary survey for naturally occurring mutations in three geographically interconnected populations of C. intestinalis. The influence of historical, geographical and environmental factors on the distribution of abnormal phenotypes was assessed by means of 12 microsatellites. We identified 37 possible mutant loci with stereotyped defects in embryonic development that segregate in a way typical of recessive alleles. Local populations were found to differ in genetic organization and frequency distribution of phenotypic classes. Natural genetic polymorphism of C. intestinalis constitutes a valuable source of phenotypes for studying embryonic development in ascidians. Correlating genetic structure and the occurrence of abnormal phenotypes is a crucial focus for understanding the selective forces that shape natural finite populations, and may provide insights of great importance into the evolutionary mechanisms that generate animal diversity.
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影响因子：
3.4
作者：
Brown, ER;Nishino, A;Okamura, Y
通讯作者：
Okamura, Y
影响因子：
4.4
作者：
Caputi, Luigi;Borra, Marco;Andreakis, Nikos;Biffali, Elio;Sordino, Paolo
通讯作者：
Sordino, Paolo
影响因子：
1.5
作者：
Deschet, K;Nakatani, Y;Smith, WC
通讯作者：
Smith, WC
DOI：
10.1098/rstb.1931.0006
发表时间：
1931-12-01
影响因子：
--
作者：
Berrill, NJ
通讯作者：
Berrill, NJ
影响因子：
7
作者：
Boffelli, D;Weer, CV;Rubin, EM
通讯作者：
Rubin, EM