Screening dataset of food components that enhance transcriptional activity of PGC1-beta
Screening dataset of food components that enhance transcriptional activity of PGC1-beta
复制标题
增强PGC1-β转录活性的食物成分筛选数据集
DOI：
10.1016/j.dib.2019.103814
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发表时间：
2019
期刊：
影响因子：
1.2
通讯作者：
Kamei Yasutomi
中科院分区：
文献类型：
--
作者：
Nakai Shiho;Uchitomi Ran;Matsuda Rintaro;Onishi Takumi;Hatazawa Yukino;Kamei Yasutomi
PGC-1β是核受体的转录共激活因子，其作用是增加能量消耗。转染HEK293T细胞后，融合到GAL4 dna结合域的PGC-1β显示出报告荧光素酶活性。我们使用报告基因分析系统筛选食物来源和天然化合物来测量PGC-1β的转录活性。我们发现大豆异黄酮、染料木黄酮和大豆黄酮以及几种白藜芦醇可以激活PGC-1β，见“染料木黄酮、大豆黄酮和白藜芦醇刺激PGC-1β介导的基因表达”[1]。166种化合物及其报告活性如下图所示。
PGC-1β is a transcriptional co-activator of nuclear receptors, which acts to increase energy expenditure. PGC-1β fused to GAL4 DNA-binding domain transfected in HEK293T cells showed a reporter luciferase activity. We screened food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β.We found that soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1β, see “Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression” [1]. The list of 166 compounds and their reporter activity is shown here.