Increased In Vivo Glucose Recovery via Nitric Oxide Release
Increased In Vivo Glucose Recovery via Nitric Oxide Release
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DOI：
10.1021/ac103070t
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发表时间：
2011-02-15
影响因子：
7.4
通讯作者：
Schoenfisch, Mark H.
中科院分区：
文献类型：
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作者：
Nichols, Scott P.;Le, Nga N.;Schoenfisch, Mark H.
在大鼠模型中，使用饱和NO溶液稳定释放NO，评价皮下植入的释放一氧化氮（NO）的微透析探针的体内葡萄糖恢复。这种方法导致每天灌流8小时内探针膜的恒定NO通量为162 pmol cm（-2）s（-1）。通过在14天的时间内监测葡萄糖恢复来评估增强的局部NO的体内作用，此后进行组织学分析。相对于对照，从7天开始观察到释放NO的探针的葡萄糖恢复的差异。第14天的组织学分析显示，探针表面的炎性细胞密度减少，包膜厚度降低。总的来说，这些结果表明，植入物表面间歇性持续释放NO可能会通过减轻异物反应来改善皮下植入传感器的葡萄糖扩散。
The in vivo glucose recovery of subcutaneously implanted nitric oxide (NO)-releasing microdialysis probes was evaluated in a rat model using saturated NO solutions to steadily release NO. Such methodology resulted in a constant NO flux of 162 pmol cm(-2) s(-1) from the probe membrane over 8 h of perfusion daily. The in vivo effects of enhanced localized NO were evaluated by monitoring glucose recovery over a 14 day period, with histological analysis thereafter. A difference in glucose recovery was observed starting at 7 days for probes releasing NO relative to controls. Histological analysis at 14 days revealed lessened inflammatory cell density at the probe surface and decreased capsule thickness. Collectively, the results suggest that intermittent sustained NO release from implant surfaces may improve glucose diffusion for subcutaneously implanted sensors by mitigating the foreign body reaction.