Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
Cell-specific thioautotrophic productivity of epsilon-proteobacterial epibionts associated with Shinkaia crosnieri.
复制标题
与Shinkaia crosnieri相关的Epsilon-细菌表皮的细胞特异性硫代营养生产力。
DOI：
10.1371/journal.pone.0046282
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Takai K
中科院分区：
文献类型：
--
作者：
Watsuji TO;Nishizawa M;Morono Y;Hirayama H;Kawagucci S;Takahata N;Sano Y;Takai K
在这项研究中，我们报告了与日本冲绳海槽深海热液系统特有的新海蟹属(Shinkaia Crosnieri)刚毛相关的共生微生物群落硫代自养活性的实验证据。在原位静压和常压条件下，微生物对还原硫化物的消耗提供了共生微生物群落氧化硫磺活性的证据；在原位和减压条件下，硫磺的氧化速率相似。微生物对还原硫化物的消耗和13C标记碳酸氢盐在硫代硫酸盐(用作硫代自养底物)存在和不存在的情况下的示踪实验结果令人信服地证明，克罗斯尼埃链霉菌上的共生微生物群落通过与还原硫化合物氧化耦合的能量代谢来驱动初级生产。示踪实验、荧光原位杂交(FISH)和纳米级二次离子质谱仪(Nano-SIMS)相结合的结果表明，属于变形杆菌纲的硫杆菌属的丝状细胞是克氏葡萄球菌共生群落中的硫代自养细胞。综上所述，我们的结果有力地表明，作为共生微生物群落的Sulfurovum成员的硫代自养生产在可能的营养外生共生中发挥了主导作用。
In this study, we report experimental evidence of the thioautotrophic activity of the epibiotic microbial community associated with the setae of Shinkaia crosnieri, a galatheid crab that is endemic to deep-sea hydrothermal systems in the Okinawa Trough in Japan. Microbial consumption of reduced sulfur compounds under in situ hydrostatic and atmospheric pressure provided evidence of sulfur-oxidizing activity by the epibiotic microbial community; the rate of sulfur oxidation was similar under in situ and decompressed conditions. Results of the microbial consumption of reduced sulfur compounds and tracer experiments using 13C-labeled bicarbonate in the presence and absence of thiosulfate (used as a thioautotrophic substrate) convincingly demonstrated that the epibiotic microbial community on S. crosnieri drove primary production via an energy metabolism that was coupled with the oxidation of reductive sulfur compounds. A combination of tracer experiments, fluorescence in situ hybridization (FISH) and nano-scale secondary ion mass spectrometry (Nano-SIMS) indicated that the filamentous cells of the genus Sulfurovum belonging to the class Epsilonproteobacteria were thioautotrophs in the epibiotic community of S. crosnieri. In conclusion, our results strongly suggest that thioautotrophic production by Sulfurovum members present as the epibiotic microbial community play a predominant role in a probable nutritional ectosymbiosis with S. crosnieri.