The functional regeneration of syncytiotrophoblast in cultured explants of term placenta
The functional regeneration of syncytiotrophoblast in cultured explants of term placenta
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DOI：
10.1152/ajpregu.2001.280.4.r1116
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发表时间：
2001-04-01
影响因子：
2.8
通讯作者：
Greenwood, SL
中科院分区：
文献类型：
--
作者：
Simán, CM;Sibley, CP;Greenwood, SL
我们研究了长期（7-11天）培养的足月人胎盘绒毛外植体的功能特征。将胎盘绒毛组织碎片（类似于5 -10 mg湿重）在补充的CMRL-1066培养基中培养长达11天。培养第一天后，合体滋养细胞出现空泡化并最终退化。然而，一个新的合胞体滋养层开发的第4天，是无法区分的新鲜胎盘的第11天。随着培养时间的延长，培养液中人绒毛膜促性腺激素的释放增加，乳酸脱氢酶活性下降。在培养的前7天测量运输变量。Rb-86的基础外排随培养时间的延长而减少，并被Ba ~（2+）抑制，提示Rb-86的基础外排是由K ~+通道介导的。在培养的所有阶段，Rb-86流出刺激ATP，低渗培养基，和ANG II。一个复杂的模式外排的变化与文化的时间和类型的刺激剂进行了观察，这表明，几个隔间的组织有助于刺激外排。这种培养系统为研究胎盘功能的慢性调节提供了机会。
We have investigated the functional characteristics of term human placental villous explants kept in long-term (7-11 days) culture. Fragments of placental villous tissue ( similar to5-10 mg wet wt) were cultured in supplemented CMRL-1066 culture medium for up to 11 days. After the first day of culture, the syncytiotrophoblast appeared vacuolated and eventually degenerated. However, a new syncytiotrophoblast developed by day 4, being indistinguishable from that of a fresh placenta by 11 days. Release of human chorionic gonadotrophin increased and activity of lactate dehydrogenase in culture medium decreased with culture time. Transport variables were measured over the first 7 days of culture. Basal Rb-86 efflux was reduced with time in culture and was inhibited by Ba2+, suggesting the efflux was mediated by K+ channels. At all stages of culture, Rb-86 efflux was stimulated by ATP, hyposmotic medium, and ANG II. A complex pattern of efflux changes with culture time and type of stimulator was observed, suggesting that several compartments of the tissue contributed to stimulated efflux. This culture system provides opportunities for studies of chronic regulation of placental function.