A fluorogenic probe for dynamic tracking of lipid droplets’ polarity during the evolution of cancer
A fluorogenic probe for dynamic tracking of lipid droplets’ polarity during the evolution of cancer
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DOI：
10.1039/d0nj05900e
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发表时间：
2021-03
影响因子：
3.3
通讯作者：
Cong Liu;Junling Yin;Bingli Lu;Weiying Lin
中科院分区：
文献类型：
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作者：
Cong Liu;Junling Yin;Bingli Lu;Weiying Lin
研究肿瘤细胞内脂滴极性的变化对肿瘤的检测和治疗具有重要意义。因此，我们设计并合成了一种新型的极性敏感荧光探针CTPE，该探针以香豆素为极性敏感基团，四苯基乙烯为供电子基团靶向ld。由于其特殊的分子内电荷转移机制，CTPE在高极性溶剂中发出弱的绿色荧光，在低极性溶剂中发出强的绿色荧光，因此荧光信号随溶剂极性的变化会发生剧烈的变化。利用探针CTPE，已经成功地从正常组织中识别出肿瘤组织，并且可以随着癌症的发展揭示ld极性的降低。此外，在组织水平上观察到药物治疗肿瘤中ld极性的变化，赋予其抗癌药物疗效评价的能力。这项工作为癌症研究和临床诊断中的ld跟踪提供了一种有前途的成像工具。
Exploring the changes in the polarity of intracellular lipid droplets (LDs) during the evolution of cancer is important for cancer detection and treatment. Therefore, we designed and synthesized a new type of polarity-sensitive fluorescent probe, CTPE, which uses coumarin as the polarity-sensitive group and tetraphenylethylene as the electron-donating group to target LDs. Due to its special intramolecular charge transfer mechanism, CTPE emitted a weak green fluorescence in high-polar solvents, and a strong green fluorescence in low-polar solvents, thus exhibiting a drastic change in fluorescence signal as a function of the solvent polarity. In virtue of the probe CTPE, tumorous tissues have been successfully identified from normal ones and the decrease in LDs’ polarity could be revealed with the evolution of cancer. Moreover, the changes in the LDs’ polarity in drugs-treated tumor were observed at the tissue level, endowing its capacity for anti-cancer drug efficacy evaluation. This work provides a promising imaging tool for LDs tracking in cancer research and clinical diagnosis.