Molecular biology of Porcine circovirus:: analyses of gene expression and viral replication
Molecular biology of Porcine circovirus:: analyses of gene expression and viral replication
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DOI：
10.1016/j.vetmic.2003.10.014
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发表时间：
2004-02-04
影响因子：
3.3
通讯作者：
Finsterbusch, T
中科院分区：
文献类型：
--
作者：
Mankertz, A;Çaliskan, R;Finsterbusch, T
猪圆环病毒I型的rep基因指导两种蛋白质的合成。全长蛋白质Rep的大小为312个氨基酸，剪接变体Rep'被截短（168个氨基酸），外显子2被移码。PCV1 DNA的复制依赖于两种蛋白质的合成。Rep和Rep'在体外与包含PCV 1复制起点部分的双链DNA片段结合，但两种蛋白的最小结合位点不同。Rep蛋白通过与两个内部六聚体H1和H2结合来抑制Rep基因的启动子。尽管Rep'与相同的序列结合，但它不影响Prep。PCV1感染后12小时，通过实时PCR检测到相似量的rep和rep'，但随后，两种转录本的比例发生变化。这两种蛋白质是共同定位在细胞核和形成的同源和异源复合物已被观察到。当进行复制测定时，其中使用PCV 1的Rep和Rep'蛋白来复制PCV 1和PCV 2的起点，发现rep基因产物在两个复制起点处启动复制。(C)2003 Elsevier B.V.保留所有权利。
The rep gene of Porcine circovirus type I directs the synthesis of two proteins. The full-length protein Rep is 312 amino acids in size, the spliced variant Rep' is truncated (168 aa) and exon 2 is frame-shifted. Replication of PCV1 DNA depends on synthesis of both proteins. Rep and Rep' bind in vitro to double-stranded DNA fragments comprising part of the origin of replication of PCV1, but the minimal binding sites of the two proteins are distinct. Rep protein represses the promoter of the rep gene by binding to the two inner hexamers HI and H2. Although Rep' binds to the same sequence, it does not influence Prep. Twelve hours after PCV1 infection, similar amounts of rep and rep' were detected by real-time PCR, but later on, the ratio of the two transcripts varied. Both proteins are co-localised in the nucleus and formation of homo- and heteromeric complexes has been observed. When a replication assay was performed, in which Rep and Rep' protein of PCV1 was used to replicate the origin of PCV1 and PCV2, the rep gene products were found to initiate replication at both origins of replication. (C) 2003 Elsevier B.V. All rights reserved.