A novel and sensitive chemiluminescence immunoassay based on AuNCs@pepsin@luminol for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether.
A novel and sensitive chemiluminescence immunoassay based on AuNCs@pepsin@luminol for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether and tetrabromobisphenol A mono(hydroxyethyl) ether.
复制标题
DOI：
10.1016/j.aca.2018.06.039
复制
发表时间：
2018-06
影响因子：
6.2
通讯作者：
Zhen Zhang;Nuanfei Zhu;Y. Zou;Zongshan Zhao;Xiangyang Wu;Guoxi Liang;Zhixiang Han;Hui Meng
中科院分区：
文献类型：
--
作者：
Zhen Zhang;Nuanfei Zhu;Y. Zou;Zongshan Zhao;Xiangyang Wu;Guoxi Liang;Zhixiang Han;Hui Meng
建立了一种基于鲁米诺修饰金纳米团簇（AuNCs@Peps@luminol）的新型化学发光免疫分析法，用于同时检测四溴双酚A （TBBPA）的重要衍生物和副产物四溴双酚A单（2-羟乙基）醚（TBBPA- dhee）和四溴双酚A单（羟乙基）醚（TBBPA- mhee）。在该系统中，碱性磷酸酶（ALP）被标记在第二抗体（Ab2）上进行信号扩增。当ALP- ab2被抗原-一抗（Ab1）复合物捕获时，苯磷酸二钠（PPNa）在ALP的催化下产生大量苯酚，显著抑制AuNCs@Peps@luminol的化学发光强度。在优化条件下，TBBPA-DHEE的计算检出限（LOD， 90%抑制）为0.078 μg/L，线性范围为0.23 ~ 9.32 μg/L，比同一抗体的常规ELISA低9倍。该方法具有良好的准确度和精密度（回收率为88.00 ~ 113.4%；CV为2.75 ~ 8.14%），可用于环境和食品样品中痕量TBBPA-DHEE和TBBPA-MHEE的浓度测定。
A novel chemiluminescence immunoassay based on luminol-modified gold nanoclusters (AuNCs@Peps@luminol) was developed for simultaneous detection of tetrabromobisphenol A bis(2-hydroxyethyl) ether (TBBPA-DHEE) and tetrabromobisphenol A mono(hydroxyethyl) ether (TBBPA-MHEE), an important derivative and byproduct of tetrabromobisphenol A (TBBPA), respectively. In the system, alkaline phosphatase (ALP) was labeled on the second antibody (Ab2) for signal amplification. When ALP-Ab2was captured by antigen-primary antibody (Ab1) complex, disodium phenyl phosphate (PPNa) generated massive phenol under the catalysis of ALP, markedly inhibiting the chemiluminescence intensity of AuNCs@Peps@luminol. Under the optimized conditions, the calculated detection of limit (LOD, 90% inhibition) was 0.078 μg/L for TBBPA-DHEE with a linear range of 0.23–9.32 μg/L, which was lower 9 times than that of conventional ELISA with the same antibody. In addition, our method showed satisfactory accuracy and precision (recoveries, 88.00–113.4%; CV, 2.75–8.14%), it can be applied to systematically investigate the concentration of the trace TBBPA-DHEE and TBBPA-MHEE in environmental and food samples.