Online data analysis at the ESRF bioSAXS beamline, BM29
Online data analysis at the ESRF bioSAXS beamline, BM29
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DOI：
10.1107/s1600576715024462
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发表时间：
2016-02-01
影响因子：
6.1
通讯作者：
Round, Adam
中科院分区：
文献类型：
--
作者：
Brennich, Martha Elisabeth;Kieffer, Jerome;Round, Adam
在同步辐射源上的高通量小角度X射线散射（bioSAXS）是结构生物学中常用的技术，其依赖于高度自动化的数据采集。bioSAXS实验的数据简化和初步分析包括一系列明确定义的单独任务，其自动化允许对收集的数据的质量进行首次简单评估，并在必要时调整收集策略。本文描述了使用EDNA框架在ESRF BM 29光束线上自动处理bioSAXS数据的逻辑和技术实现。
High-throughput small-angle X-ray scattering on proteins in solution (bioSAXS) at synchrotron sources is a commonly used technique in structural biology, which relies on highly automated data acquisition. Data reduction and primary analysis for bioSAXS experiments consist of a well defined series of individual tasks, the automation of which allows a first easy assessment of the quality of collected data and the adjustment of collection strategies if necessary. This article describes both the logic and the technical implementation of the automated processing pipeline for bioSAXS data at the ESRF BM29 beamline using the EDNA framework.