The kinetics of the hypoferraemic response and changes in levels of alternative complement activity in diploid and triploid Atlantic salmon, following injection of lipopolysaccharide
The kinetics of the hypoferraemic response and changes in levels of alternative complement activity in diploid and triploid Atlantic salmon, following injection of lipopolysaccharide
复制标题
DOI：
10.1006/fsim.2000.0319
复制
发表时间：
2001-05-01
影响因子：
4.7
通讯作者：
Ellis, AE
中科院分区：
文献类型：
--
作者：
Langston, AL;Johnstone, R;Ellis, AE
为了研究三倍体对两种非特异性疾病因子的反应动力学的任何可能的影响，将脂多糖（1 mg kg（-1）体重）或盐水腹腔内注射到完全同胞二倍体和三倍体大西洋鲑鱼中。个别标记的鱼重复血液采样长达19天。在整个实验过程中，总血清蛋白浓度保持恒定，表明采样方案不会导致血液稀释。在注射盐水的鱼的血清中的补体旁路途径活性（通过对兔红细胞的溶血活性的滴度测量）保持恒定，但在注射LPS的鱼中，其在注射后2天下降至几乎不可检测的水平，但在约第5天恢复至处理前水平。三倍体鱼达到完全恢复水平所需的时间比二倍体稍长。所有组的鱼表现出低铁血症的反应，这表明采样制度至少是部分负责。然而，在注射LPS的鱼中，反应更迅速和明显。在后者中，血清铁浓度下降到非常低的水平，由第2天注射后的二倍体鱼和第3天的三倍体鱼。在所有组中，在注射后约15天重新建立治疗前铁水平。数据显示，二倍体和三倍体鱼之间只有轻微的差异，但三倍体恢复补体活性所需的时间较长，注射LPS后低铁血症反应的发生较慢，这表明它们可能处于不利地位，与它们的二倍体同胞相比，在抵御细菌感染。(C)北京：科学出版社.
To study any possible effects of triploidy on the kinetics of the response of two non-specific disease factors, full sibling diploid and triploid Atlantic salmon were injected intraperitoneally with either lipopolysaccharide (1 mg kg(-1) body weight) or saline. Individually marked fish were repetitively blood sampled for up to 19 days. Total serum protein concentrations remained constant throughout the experiment indicating that the sampling regime did not cause haemodilution. The alternative complement pathway activity (measured by the titre of haemolytic activity against rabbit erythrocytes) in the serum of saline injected fish remained constant but in LPS-injected fish it fell to barely detectable levels 2 days after injection, but recovered to pre-treatment levels by about day 5. Triploid fish took slightly longer to reach full recovery levels than diploids. All groups of fish showed a hypoferraemic response, suggesting that the sampling regime was at least partially responsible. However, the response was more rapid and pronounced in the LPS-injected fish. In the latter, serum iron concentrations decreased to very low levels by day 2 post-injection in the diploid fish and by day 3 in the triploid fish. Pre-treatment iron levels were re-established by about 15 days postinjection in all groups. The data show only slight differences between the diploid and triploid fish, but the longer time taken for the triploids to recover complement activity and the slower onset of the hypoferraemic response following injection of LPS, suggest that they may be at a disadvantage compared with their diploid siblings in their defence against bacterial infections. (C) 2001 Academic Press.