Transport and localization of the DEG/ENaC ion channel BNaC1α to peripheral mechanosensory terminals of dorsal root ganglia neurons
Transport and localization of the DEG/ENaC ion channel BNaC1α to peripheral mechanosensory terminals of dorsal root ganglia neurons
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DOI：
10.1523/jneurosci.21-08-02678.2001
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发表时间：
2001-04-15
影响因子：
5.3
通讯作者：
Corey, DR
中科院分区：
文献类型：
--
作者：
García-Añoveros, J;Samad, TA;Corey, DR
哺乳动物脑钠通道（BNaC，也称为BNC/ASIC）蛋白形成与推定的机械感觉通道相关的酸敏感性和阿米洛利可阻断的钠通道。某些BNaC同种型仅在背根神经节（DRG）中表达，并且已经提出形成介导组织酸中毒诱导的疼痛的离子通道。与抗体标记，我们发现，BNaC 1 α亚型表达的大多数大DRG神经元（低阈值机械感受器不参与酸诱导的伤害性感受）和一些小的伤害感受器神经元（其中包括高阈值机械感受器）。BNaC 1 α从DRG细胞体转运到外周的感觉末梢，但不转运到脊髓，并且特异性地位于专门的皮肤机械感觉末梢，包括Meissner、默克尔、毛状、网状、披针形和毛囊栅栏以及一些表皮内和游离的有髓鞘神经末梢。因此，BNaC 1 α通道可能参与触觉和疼痛机械刺激的转导。
Mammalian brain sodium channel (BNaC, also known as BNC/ ASlC) proteins form acid-sensitive and amiloride-blockable sodium channels that are related to putative mechanosensory channels. Certain BNaC isoforms are expressed exclusively in dorsal root ganglia (DRG) and have been proposed to form the ion channels mediating tissue acidosis-induced pain. With antibody labeling, we find that the BNaC1 alpha isoform is expressed by most large DRG neurons (low-threshold mechanosensors not involved in acid-induced nociception) and few small nociceptor neurons (which include high-threshold mechanoreceptors). BNaC1 alpha is transported from DRG cell bodies to sensory terminals in the periphery, but not to the spinal cord, and is located specifically at specialized cutaneous mechanosensory terminals, including Meissner, Merkel, penicillate, reticular, lanceolate, and hair follicle palisades as well as some intraepidermal and free myelinated nerve endings. Accordingly, BNaC1 alpha channels might participate in the transduction of touch and painful mechanical stimuli.