Noggin blocks osteoinductive activity of porcine enamel extracts
Noggin blocks osteoinductive activity of porcine enamel extracts
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DOI：
10.1177/154405910208100606
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发表时间：
2002-06-01
影响因子：
7.6
通讯作者：
Oida, S
中科院分区：
文献类型：
--
作者：
Iwata, T;Morotome, Y;Oida, S
牙釉质提取物诱导生物矿化，例如成骨和牙骨质形成，但负责这种活动的分子成分仍不确定。我们从发育中的猪牙齿中分离出牙釉质提取物并分离出骨诱导部分。在碱性条件（pH 10.8）下提取猪牙釉质刮屑中的蛋白质，并使用 Sephadex G-100（尺寸排阻）柱进行分级。在 ST2 细胞（一种小鼠骨髓基质细胞系）中测定了每种组分增强碱性磷酸酶 (ALP) 活性的能力。在组分 44 和 45 中发现了牙釉质提取物的骨诱导组分 (OFE)，其诱导 ST2 细胞表达成骨成骨细胞的表型，并形成矿化结节。此外，暴露于 OFE 的 ST2 细胞的 ALP 活性被 BMP 拮抗剂 noggin 降低，并且在斑点印迹分析中 OFE 与 BMP-2/4 抗体发生反应。这些结果表明 OFE 含有有助于诱导生物矿化的 BMP。
Enamel extracts induce biomineralization such as osteogenesis and cementogenesis, but the molecular component responsible for this activity remains uncertain. We fractionated enamel extracts from developing pig teeth and isolated the osteoinductive fraction. Proteins from pig enamel scrapings were extracted under alkaline conditions (pH 10.8) and fractionated with the use of a Sephadex G-100 (size exclusion) column. The ability of each fraction to enhance alkaline phosphatase (ALP) activity was assayed in ST2 cells, a mouse bone marrow stromal cell line. The osteoinductive fraction of enamel extracts (OFE) was found in fractions 44 and 45, which induced ST2 cells to express the phenotype of boneforming osteoblasts, and to form mineralized nodules. Furthermore, the ALP activity of ST2 cells exposed to OFE was reduced by noggin, an antagonist of BMPs, and OFE reacted with BMP-2/4 antibody in dot-blot analysis. These results indicate that OFE contains BMPs that contribute to the induction of biomineralization.