Role of stress response sigma factor SigG in Mycobacterium tuberculosis
Role of stress response sigma factor SigG in Mycobacterium tuberculosis
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DOI：
10.1128/jb.00511-07
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发表时间：
2008-02-01
影响因子：
3.2
通讯作者：
Bishai, William R.
中科院分区：
文献类型：
--
作者：
Lee, Jong-Hee;Geiman, Deborah E.;Bishai, William R.
通过同源重组打乱结核分枝杆菌的SIgG基因，并通过实时荧光定量逆转录聚合酶链式反应和基因芯片检测SIgG基因的调控。SIGG一致启动子识别序列被鉴定为GCGNGT-N15-18-CGANCA。Delta Sigg突变体对丝裂霉素C的抗性高于野生型菌株，这表明它可能参与了结核分枝杆菌的SOS反应。
The sigG gene of Mycobacterium tuberculosis was disrupted by homologous recombination, and the genes regulated by SigG were examined by real-time reverse-transcription PCR and microarray studies. The SigG consensus promoter recognition sequence was identified as GCGNGT-N15-18-CGANCA. A Delta sigG mutant was found to be more resistant to mitomycin C treatment than the wild-type strain, indicating that it may be involved in the SOS response in M. tuberculosis.