The protein phosphatase 1 regulator PNUTS is a new component of the DNA damage response
The protein phosphatase 1 regulator PNUTS is a new component of the DNA damage response
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DOI：
10.1038/embor.2010.134
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发表时间：
2010-11-01
期刊：
影响因子：
7.7
通讯作者：
Kuntziger, Thomas
中科院分区：
文献类型：
--
作者：
Landsverk, Helga B.;Mora-Bermudez, Felipe;Kuntziger, Thomas
The function of protein phosphatase 1 nuclear-targeting subunit (PNUTS)-one of the most abundant nuclear-targeting subunits of protein phosphatase 1 (PP1c)-remains largely uncharacterized. We show that PNUTS depletion by small interfering RNA activates a G2 checkpoint in unperturbed cells and prolongs G2 checkpoint and Chk1 activation after ionizing-radiation-induced DNA damage. Overexpression of PNUTS-enhanced green fluorescent protein (EGFP)-which is rapidly and transiently recruited at DNA damage sites-inhibits G2 arrest. Finally, gamma H2AX, p53-binding protein 1, replication protein A and Rad51 foci are present for a prolonged period and clonogenic survival is decreased in PNUTS-depleted cells after ionizing radiation treatment. We identify the PP1c regulatory subunit PNUTS as a new and integral component of the DNA damage response involved in DNA repair.
