Brucella melitensis M5-90 Delta bp26 as a potential live vaccine that allows for the distinction between natural infection and immunization
Brucella melitensis M5-90 Delta bp26 as a potential live vaccine that allows for the distinction between natural infection and immunization
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布鲁氏菌 M5-90 Delta bp26 作为一种潜在的活疫苗，可以区分自然感染和免疫
DOI：
10.1139/cjm-2017-0179
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发表时间：
2017
影响因子：
2.8
通讯作者：
Chen Chuangfu
中科院分区：
文献类型：
--
作者：
Li Tiansen;Tong Zhixia;Huang Meiling;Tang Liyan;Zhang Hui;Chen Chuangfu
布鲁氏菌是革兰氏阴性细胞内细菌病原体，感染人类和动物，并在发展中国家造成巨大的经济损失。目前，布鲁氏菌减毒活疫苗（羊种布鲁氏菌M5 -90）是控制和预防动物布鲁氏菌病最有效的手段。然而，这些疫苗有几个缺点，如无法区分自然感染和免疫接种，以及与妊娠动物流产的关联。因此，本研究构建了布氏杆菌M5 -90Δ bp 26突变体并评价其毒力。M5-90Δ bp 26突变体在人胎盘滋养层8细胞（HPT-8细胞）和BALB/c小鼠中的存活减弱，在小鼠中观察到高免疫保护性。此外，安全性试验表明，M5-90Δ bp 26突变株的毒力低于M5-90疫苗株。此外，采用间接酶联免疫吸附试验（ELISA）检测布鲁氏菌蛋白26（BP 26）的存在，具有较高的灵敏度，而M5-90Δ bp 26的接种伴随BP 26的缺失表达，并进一步通过蛋白质印迹法得到证实。M5-90Δ bp 26突变体和间接ELISA可用于区分接种疫苗的牲畜和感染的动物。
Brucellais Gram-negative intracellular bacterial pathogen that infects humans and animals and contributes to great economic losses in developing countries. Presently, live attenuatedBrucellavaccines (Brucella melitensisM5-90) are the most effective means of brucellosis control and prevention in animals. However, these vaccines have several drawbacks, such as an inability to distinguish between a natural infection and immunization and an association with abortions in pregnant animals. Therefore, this study constructed aBrucellaM5-90Δbp26 mutant and evaluated its virulence. The survival of the M5-90Δbp26 mutant was attenuated in human placenta trophoblastic 8 cells (HPT-8 cells) and in BALB/c mice, with a high immunoprotectivity noted in mice. Furthermore, safety tests showed that the M5-90Δbp26 mutant was less virulent than the M5-90 vaccine strain. Additionally, an indirect enzyme-linked immunosorbent assay (ELISA) screening was shown to detect the presence ofBrucellaprotein 26 (BP26) with high sensitivity, with M5-90Δbp26 inoculation accompanied with a lack of BP26 expression, and was further confirmed by western blotting. Together, the M5-90Δbp26 mutant and the indirect ELISA can be employed to distinguish vaccinated livestock from infected animals.