Activin A inhibits differentiation of 3T3-L1 preadipocyte
Activin A inhibits differentiation of 3T3-L1 preadipocyte
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DOI：
10.1016/j.mce.2005.01.001
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发表时间：
2005-03-31
影响因子：
4.1
通讯作者：
Yano, H
中科院分区：
文献类型：
--
作者：
Hirai, S;Yamanaka, M;Yano, H
本实验研究了激活素A对13 T3-L1前脂肪细胞分化的影响。激活素A抑制诱导终末分化标志物，如甘油-3-磷酸脱氢酶（GPDH）活性，脂质积累，和脂肪细胞脂肪酸结合蛋白（O-2）mRNA的表达时，细胞与激活素A处理整个分化期。在早期阶段，激活素A处理降低GPDH活性和aP 2 mRNA水平，并且还降低过氧化物酶体增殖物激活受体（PPAR）γ和CCAAT/增强子结合蛋白（C/EBP）α mRNA的表达，而不影响C/EBPP活性亚型及其mRNA的表达。另一方面，激活素A处理对有丝分裂克隆扩增没有影响。这些结果表明激活素A通过影响PPAR γ表达上游的转录因子级联来抑制脂肪形成。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
We investigated the effect of activin A on differentiation of 13T3-L1 preadipocyte. Activin A suppressed the induction of terminal differentiation markers such as glycerol-3-phosphate dehydrogenase (GPDH) activity, lipid accumulation, and the expression of adipocyte fatty acid-binding protein (O-2) mRNA when the cells were treated with activin A throughout the differentiation period. Activin A treatment during the early phase decreased GPDH activity and aP2 mRNA level, and also reduced the expression of peroxisome proliferator-activated receptor (PPAR) gamma and CCAAT/enhancer binding protein (C/EBP) a mRNAs without affecting the expressions of the active isoforms of C/EBPP and its mRNA. On the other hand, activin A treatment had no effect on the mitotic clonal expansion. These results indicate that activin A inhibits adipogenesis via affecting the transcriptional factor cascade upstream of PPAR gamma expression. (c) 2005 Elsevier Ireland Ltd. All rights reserved.