Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos.
Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos.
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DOI：
10.2527/jas.2005-695
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发表时间：
2006-08
影响因子：
3.3
通讯作者：
T. Suteevun;T. Suteevun;R. Parnpai;Sadie L. Smith;Chang Cc;S. Muenthaisong;X. Tian
中科院分区：
文献类型：
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作者：
T. Suteevun;T. Suteevun;R. Parnpai;Sadie L. Smith;Chang Cc;S. Muenthaisong;X. Tian
由于繁殖效率低下，沼泽水牛正变得濒临灭绝。这是令人担忧的，因为许多国家严重依赖其产品。体细胞核移植（SCNT）是保护濒危物种的潜在策略。迄今为止，SCNT在沼泽水牛身上已成功培育出囊胚胚胎。然而，SCNT 沼泽水牛的足月发育极为有限，仅报道过 1 头活产。异常的表观遗传机制被怀疑是发育失败的原因，这在其他物种中也是如此。 DNA 甲基化和组蛋白乙酰化是表观遗传修饰的关键因素，并在胚胎植入前发育过程中表现出显着的变异性。表观遗传修饰的知识将有助于解决 SCNT 胚胎的发育问题并改善沼泽水牛的繁殖技术。本研究的目的是确定 SCNT 和体外受精 (IVF) 沼泽水牛胚胎中植入前胚胎发育与 2 种表观遗传模式（整体 DNA 甲基化和组蛋白乙酰化）之间的关系。此外，我们使用双免疫染色和共聚焦显微镜量化发射强度，检查了 SCNT 和 IVF 沼泽水牛胚胎在整个发育阶段中这两种机制之间的相关性。我们在早期植入前阶段沼泽水牛 SCNT 胚胎中发现了异常的甲基化模式。此外，还发现 SCNT 胚胎内细胞核之间 DNA 甲基化水平存在更大的变异性。与 4 细胞和 8 细胞阶段的 IVF 胚胎相比，SCNT 胚胎中也观察到了高度乙酰化（P < 0.05）。这两种表观遗传机制之间的动态变化和相互作用对于植入前早期的胚胎发育至关重要。这里发现的异常可能导致 SCNT 效率低下。
Swamp buffalos are becoming endangered due to reproductive inefficiencies. This is of concern because many countries depend heavily on their products. Somatic cell nuclear transfer (SCNT) is a potential strategy for preserving endangered species. To date, SCNT in swamp buffalo has succeeded in the creation of blastocyst embryos. However, development to term of SCNT swamp buffalos is extremely limited, and only 1 live birth has been reported. An abnormal epigenetic mechanism is suspected to be the cause of developmental failure, as is also seen in other species. The DNA methylation and histone acetylation are key players in epigenetic modification and display marked variability during embryonic preimplantation development. Knowledge of epigenetic modifications will aid in solving the developmental problems of SCNT embryos and improving reproductive technology in the swamp buffalo. The objective of this study was to determine the relationship between preimplantation embryonic development and 2 epigenetic patterns, global DNA methylation and histone acetylation, in SCNT and in vitro-fertilized (IVF) swamp buffalo embryos. In addition, we examined the correlations between those 2 mechanisms in the SCNT and IVF swamp buffalo embryos throughout the developmental stages using double immunostaining and quantification of the emission intensities using confocal microscopy. We discovered an aberrant methylation pattern in early preimplantation-stage swamp buffalo SCNT embryos. In addition, greater variability in the DNA methylation levels among nuclei within SCNT embryos was discovered. Hyperacetylation was also observed in SCNT embryos compared with IVF embryos at the 4- and 8-cell stages (P < 0.05). Dynamic changes and interplay between these 2 epigenetic mechanisms could be crucial for embryonic development during the early preimplantation period. The aberrancies uncovered here may contribute to the low efficiency of SCNT.