A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica
A simple method for DNA extraction from sporophyte in the brown alga Laminaria japonica
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DOI：
10.1007/s10811-005-5557-9
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发表时间：
2005
影响因子：
3.3
通讯作者：
Gaoge Wang;Yuhui Li;Peng Xia;D. Duan
中科院分区：
文献类型：
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作者：
Gaoge Wang;Yuhui Li;Peng Xia;D. Duan
A simple method was developed for extracting DNA from brown algaeLaminaria japonica, which possess large amounts of acidic polysaccharides. Firstly, the sporophyte were washed by eliminating polysaccaride buffer to remove the polysaccharides and then ground in liquid nitrogen. Secondly, the powders were treated with lysing buffer. Thirdly, KAc was used to eliminate the remaining acidic polysaccharides. The extracted DNA was purified using a chloroform-isoamyl alcohol (24:1 v/v), and precipitated in cold isopropanol. The yield was from 18.7 to 37.5 μg g−1(wet weight) and the purity of total DNA was determined spectrophotometrically as the ratio of A260/A280, which was about 1.7–1.9. The extracted DNA was of high quality and suitable for molecular analyses, such as PCR, restriction enzyme digestion. This method is a reproducible, simple, and rapid technique for routine DNA extraction from sporophyte inLaminaria japonica. Furthermore, the low cost of this method makes it attractive for large-scale studies.
