NAP1 Family Histone Chaperones Are Required for Somatic Homologous Recombination in Arabidopsis
NAP1 Family Histone Chaperones Are Required for Somatic Homologous Recombination in Arabidopsis
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拟南芥体细胞同源重组需要 NAP1 家族组蛋白伴侣
DOI：
10.1105/tpc.112.096792
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发表时间：
2012-04-01
期刊：
影响因子：
11.6
通讯作者：
Shen, Wen-Hui
中科院分区：
文献类型：
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作者：
Gao, Juan;Zhu, Yan;Shen, Wen-Hui
同源重组（HR）是维持基因组完整性和可变性所必需的。在染色质的背景下协调HR是一个挑战，无论是在DNA可及性和DNA修复后染色质组织的恢复方面。组蛋白伴侣蛋白参与核小体的组装/拆卸，并可能在HR中发挥作用。在这里，我们表明核小体组装蛋白1 （NAP1）家族组蛋白伴侣是拟南芥体细胞HR所必需的。在正常生长条件下以及在广泛的遗传毒性或非生物胁迫下，NAP1组或NAP1相关蛋白（NRP）组蛋白的缺失都会导致植物HR的降低。这与染色质组装因子-1 （caf1）组蛋白伴侣蛋白耗竭引起的高重组基因表型形成对比。此外，我们发现由ca -1缺失引起的高重组表型依赖于NRP1和NRP2，但端粒缩短表型不依赖于NRP1和NRP2。我们对DNA损伤、H3K56乙酰化和DNA修复基因表达的分析表明，NAP1家族组蛋白伴侣在HR期间核小体拆卸/重组中的作用。我们的研究强调了不同家族的组蛋白伴侣在维持基因组稳定性方面的不同功能，并建立了NAP1家族组蛋白伴侣在体细胞HR中的关键功能。
Homologous recombination (HR) is essential for maintaining genome integrity and variability. To orchestrate HR in the context of chromatin is a challenge, both in terms of DNA accessibility and restoration of chromatin organization after DNA repair. Histone chaperones function in nucleosome assembly/disassembly and could play a role in HR. Here, we show that the NUCLEOSOME ASSEMBLY PROTEIN1 (NAP1) family histone chaperones are required for somatic HR in Arabidopsis thaliana. Depletion of either the NAP1 group or NAP1-RELATED PROTEIN (NRP) group proteins caused a reduction in HR in plants under normal growth conditions as well as under a wide range of genotoxic or abiotic stresses. This contrasts with the hyperrecombinogenic phenotype caused by the depletion of the CHROMATIN ASSEMBLY FACTOR-1 (CAF-1) histone chaperone. Furthermore, we show that the hyperrecombinogenic phenotype caused by CAF-1 depletion relies on NRP1 and NRP2, but the telomere shortening phenotype does not. Our analysis of DNA lesions, H3K56 acetylation, and expression of DNA repair genes argues for a role of NAP1 family histone chaperones in nucleosome disassembly/reassembly during HR. Our study highlights distinct functions for different families of histone chaperones in the maintenance of genome stability and establishes a crucial function for NAP1 family histone chaperones in somatic HR.