A tetRA-based promoter system for the generation of conditional knockouts in Campylobacter jejuni
A tetRA-based promoter system for the generation of conditional knockouts in Campylobacter jejuni
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基于 tetRA 的启动子系统，用于在空肠弯曲杆菌中产生条件敲除
DOI：
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
M. Beeby
中科院分区：
文献类型：
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作者：
Eli J. Cohen;Rui Tong Quek;M. Beeby
空肠弯曲杆菌每年造成数千万例胃肠炎。尽管它的流行和对人类健康的影响，可供研究人员研究C。空肠仍然有限。为了扩大该物种的遗传工具包，我们设计了一个基于tetRA四环素抗性盒产生条件性敲除的系统。该系统表现出紧密的抑制性和滴定性的目标基因的表达，并将是有用的，为未来的研究这一重要的人类病原体。
Campylobacter jejuni is responsible for tens of millions of cases of gastroenteritis each year. Despite its prevalence and impact on human health, the repertoire of genetic tools available for researchers to study C. jejuni remains limited. In order to expand upon the genetic toolkit in this species, we have engineered a system for generating conditional knockouts based on the tetRA tetracycline-resistance cassette. This system exhibits tight repressibility and titratability of target-gene expression and will be useful for future research on this important human pathogen.