The Frankia alni Symbiotic Transcriptome
The Frankia alni Symbiotic Transcriptome
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DOI：
10.1094/mpmi-23-5-0593
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发表时间：
2010-05-01
影响因子：
3.5
通讯作者：
Kucho, Ken-ichi
中科院分区：
文献类型：
--
作者：
Alloisio, Nicole;Queiroux, Clothilde;Kucho, Ken-ichi
放线菌Frankia spp.能够诱导在大量放线菌植物的根部形成根瘤，在那里它们将二氮转化为氨以交换植物光合产物。在本研究中，使用全基因组微阵列，对氮充足的自由生活的Frankia did细胞和Alnus dubiosa根瘤细菌进行转录分析。根瘤诱导基因在基因组上的分布主要集中在3个Frankia spp之间的高同线性区域。而结节抑制基因，大多是假设的，不保守的，分布在基因组中。已知与固氮相关的基因被高度诱导，nif（固氮酶），hup 2（氢化酶吸收），suf（硫-铁簇）和shc（类Hopanoids合成）。参与铵同化和运输的基因的表达被强烈修改，表明细菌铵同化是有限的。还鉴定了可能在共生中发挥作用的基因，特别是涉及转录调节、信号传导过程、蛋白质药物输出、蛋白质分泌、脂多糖和肽聚糖生物合成的基因。我们还表明，这种Frankia共生转录组是高度相似的遗传远缘植物家庭桦木科和杨梅科。最后，与根瘤菌转录组的比较表明，F。在共生过程中，Did比根瘤菌代谢更活跃。
The actinobacteria Frankia spp. are able to induce the formation of nodules on the roots of a large spectrum of actinorhizal plants, where they convert dinitrogen to ammonia in exchange for plant photosynthates. In the present study, transcriptional analyses were performed on nitrogen-replete free-living Frankia did cells and on Alnus glutinosa nodule bacteria, using whole-genome microarrays. Distribution of nodule-induced genes on the genome was found to be mostly over regions with high synteny between three Frankia spp. genomes, while nodule-repressed genes, which were mostly hypothetical and not conserved, were spread around the genome. Genes known to be related to nitrogen fixation were highly induced, nif (nitrogenase), hup2 (hydrogenase uptake), suf (sulfur-iron cluster), and shc (hopanoids synthesis). The expression of genes involved in ammonium assimilation and transport was strongly modified, suggesting that bacteria ammonium assimilation was limited. Genes involved in particular in transcriptional regulation, signaling processes, protein drug export, protein secretion, lipopolysaccharide, and peptidoglycan biosynthesis that may play a role in symbiosis were also identified. We also showed that this Frankia symbiotic transcriptome was highly similar among phylogenetically distant plant families Betulaceae and Myricaceae. Finally, comparison with rhizobia transcriptome suggested that F. did is metabolically more active in symbiosis than rhizobia.