Identification of Ehrlichia spp, and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia
Identification of Ehrlichia spp, and Borrelia burgdorferi in Ixodes ticks in the Baltic regions of Russia
复制标题
DOI：
10.1128/jcm.39.6.2237-2242.2001
复制
发表时间：
2001-06-01
影响因子：
9.4
通讯作者：
Schouls, LM
中科院分区：
文献类型：
--
作者：
Alekseev, AN;Dubinina, HV;Schouls, LM
埃立克体的存在和分布。在俄罗斯波罗的海地区收集的硬蜱中证实了伯氏疏螺旋体，在那里莱姆疏螺旋体病是地方病。共采集蓖麻硬蜱标本3,426份，全沟硬蜱标本1,267份，暗视野显微镜检查结果显示，265份（11.5%）为I.蓖麻和333（26.3%）I.全沟蜱阳性。从这些样本中，对472只暗场阳性蜱和159只暗场阴性蜱进行PCR和随后的反向线印迹杂交。54只蜱（8.6%）携带埃立克体属，4只（0.6%）携带属于嗜吞噬细胞埃立克体复合体的埃立克体，包括人粒细胞埃立克体病因子。急诊嗜吞噬细胞复合体和埃立克体样种仅在I.而小鼠埃立克体（Ehrlichia muris）仅见于I.全沟，表明可能的媒介特异性感染。伽氏疏螺旋体主要分布在I.全沟疏螺旋体在两种蜱种中分布均匀，而阿氏疏螺旋体在两种蜱种中分布均匀。只有两个我。蓖麻蜱携带B。burgdorferi sensu stricto，而瓦氏疏螺旋体（Borrelia valaisiana）和一种新发现的B.在所有蜱中分别发现了1.7%和2.5%的阿氏样种。在暗视野阳性蜱中，仅64.8%产生疏螺旋体PCR产物，表明暗视野显微镜可检测到除B以外的生物体。burgdorferi sensu late.这些观察结果表明，人粒细胞埃立克体病的病原体可能存在于俄罗斯波罗的海地区的蜱虫中，临床医生应该意识到这种病原体是发热性疾病的一个原因。
The presence and distribution of Ehrlichia spp. and Borrelia burgdorferi sensu late was demonstrated among ixodid ticks collected in the Baltic regions of Russia, where Lyme borreliosis is endemic. A total of 3,426 Ixodes ricinus and 1,267 Ixodes persulcatus specimens were collected, and dark-field microscopy showed that 265 (11.5%) I. ricinus and 333 (26.3%) I. persulcatus ticks were positive. From these samples, 472 dark-field-positive and 159 dark-field-negative ticks were subjected to PCR and subsequent reverse line blot hybridization. Fifty-four ticks (8.6%) carried Ehrlichia species, and 4 (0.6%) carried ehrlichiae belonging to the Ehrlichia phagocytophila complex, which includes the human granulocytic ehrlichiosis agent. The E. phagocytophila complex and an Ehrlichia-like species were detected only in I. ricinus whereas Ehrlichia muris,vas found exclusively in I. persulcatus, indicating a possible vector-specific infection. Borrelia garinii was found predominantly in I. persulcatus, but Borrelia afzelii was evenly distributed among the two tick species. Only two I. ricinus ticks carried B. burgdorferi sensu stricto, while Borrelia valaisiana and a newly identified B. afzelii-like species were found in 1.7 and 2.5% of all ticks, respectively. Of the dark-field-positive ticks, only 64.8% yielded a Borrelia PCR product, indicating that dark-field microscopy may detect organisms other than B. burgdorferi sensu late. These observations show that the agent of human granulocytic ehrlichiosis may be present in ticks in the Baltic regions of Russia and that clinicians should be aware of this agent as a cause of febrile disease.