Sole use of sucrose in human sperm cryopreservation
Sole use of sucrose in human sperm cryopreservation
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DOI：
10.1080/01485010701225675
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Osuamkpe, C. O.
中科院分区：
文献类型：
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作者：
Hossain, A. M.;Osuamkpe, C. O.
甘油单独或与其他添加剂组合是人类精子最广泛使用和最成功的冷冻保护剂之一。甘油方法需要严格的解冻后样本洗涤以用于ART，这可能导致少精子样本的精子产量较低。本研究探讨了蔗糖用于精子冷冻保存的可行性。将蔗糖作为冷冻保护剂与将样品直接投入液氮（玻璃化）作为冷冻方法相结合。对来自正常精子和严重少精子样本的蔗糖处理精子进行快速冷冻和解冻。在冷冻前（蔗糖平衡后）以及冷冻后（解冻后）评价运动性和活力。与较高浓度（200-1000 mM）的蔗糖相比，100 mM浓度的蔗糖显示出更好的冷冻保护剂特征。蔗糖（100 mM）处理的精子在冷冻和解冻后保持较低但可接受的运动力（30%）和令人满意的存活率（60%）。蔗糖对正常精子和少精子样本的冷冻保护能力相同。蔗糖法利用微量样品的快速冷冻，从而快速冷冻、解冻，并最大限度地从样品中回收精子。
Glycerol alone or in combination with other additives is one of the most widely used and successful cryoprotectants for human sperm. The glycerol method requires rigorous post thaw sample washing for use in ART and this may lead to low sperm yield from oligospermic samples. In this study the feasibility of the use of sucrose in sperm cryopreservation was explored. Sucrose as cryoprotectant was combined with direct plunging of sample into liquid nitrogen (vitrification) as a freezing method. Sucrose treated sperm from normozoospermic and severly oligozoospermic samples underwent rapid freeze and thaw. Motility and viability were evaluated before freezing (after sucrose equilibration) as well as post freezing (after thaw). The 100mM concentration of sucrose showed better cryoprotectant features compared to that of higher concentrations (200-1000 mM). Sucrose (100mM) treated sperm maintained low but acceptable motility (30%) and satisfactory viability (60%) after freezing and thawing. The cryoprotectant capacity of sucrose for normozoospermic and oligozoospermic samples were identical. The sucrose method utilizes rapid freezing of a micro volume of sample and thus quickly freezes, thaws, and maximizes recovery of the sperm from the sample.