A Microfluidic System to Evaluate Intestinal Absorption
A Microfluidic System to Evaluate Intestinal Absorption
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DOI：
10.2116/analsci.25.1403
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发表时间：
2009-12-01
影响因子：
1.6
通讯作者：
Yoshimura, Etsuro
中科院分区：
文献类型：
--
作者：
Imura, Yuki;Asano, Yasuyuki;Yoshimura, Etsuro
肠道吸收率随所涉及物质的性质而变化。通常使用细胞培养插入物进行体外实验以评价肠吸收率。然而，这些插入物需要大量的细胞、样品和培养基，并且需要很长时间来评估。为了克服这些问题，我们开发了一种基于微芯片的系统，模仿肠道。微芯片由载玻片、渗透膜和聚二甲基硅氧烷（PDMS）片组成，其中含有通过光刻法制成的微通道; Caco-2细胞在微芯片中的膜上培养。用微量注射泵调节系统。我们进行了渗透试验;环磷酰胺可以透过肠屏障，显示出高的渗透系数，而荧光黄不能在肠壁吸收，显示出低的渗透系数。这些结果与使用传统方法获得的结果一致，这支持了我们的新系统的有效性。该系统实现了80%的电池消耗减少。
Intestinal absorption rates vary with the nature of the substances involved. In-vitro experiments with cell culture inserts are often conducted to evaluate the intestinal absorption rate. These inserts, however, require large amounts of cells, samples, and culture media, and take a long time to evaluate. To overcome these problems, we developed a microchip-based system that mimics the intestine. The microchip was composed of a glass slide, a permeable membrane, and polydimethylsiloxane (PDMS) sheets, which contained microchannels made by photolithography; Caco-2 cells were cultured on the membrane in the microchip. The system was regulated with a microsyringe pump. We conducted permeation tests; cyclophosphamide, which can permeate the intestinal barrier, displayed a high permeability coefficient and Lucifer yellow, which cannot be absorbed at the intestinal wall, displayed a low permeability coefficient. These results were consistent with those obtained using a conventional method, which supports the validity of our new system. The system realized an 80% reduction of cell consumption.