ISOLATED FIBRILS RESCUE COHESION AND DEVELOPMENT IN THE DSP MUTANT OF MYXOCOCCUS-XANTHUS
ISOLATED FIBRILS RESCUE COHESION AND DEVELOPMENT IN THE DSP MUTANT OF MYXOCOCCUS-XANTHUS
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DOI：
10.1128/jb.176.23.7190-7196.1994
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发表时间：
1994-12-01
影响因子：
3.2
通讯作者：
DWORKIN, M
中科院分区：
文献类型：
--
作者：
CHANG, BY;DWORKIN, M
黄粘球菌细胞外原纤维参与细胞内聚和细胞发育。一组社会性运动突变体Dsp不能产生细胞外原纤维；这些突变体也失去了凝聚和发育的能力。从营养野生型细胞中分离出细胞外原纤维并加入到Dsp细胞中，可以完全恢复这些细胞的粘附能力并进行正常的形态发育。因此，原纤维模仿了完整的野生型细胞进行同样的拯救的能力。原纤维的最佳内聚救援需要钙和镁离子，不需要蛋白质合成，但依赖于能量，即叠氮化钠和氰化钠阻断救援。凝聚力救援也被重氮染料刚果红阻挡。内聚拯救是属特异性的，即分离的原纤维不会引起铜绿假单胞菌、枯草芽孢杆菌、奇异变形杆菌、大肠杆菌或相关的金刺杆菌的内聚。分离原纤维对Dsp的发育修复包括聚集、子实体形成和粘孢子形态发生。分离的原纤维仅部分恢复了Dsp突变体的发育性基因表达。
Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added to Dsp cells fully restored the abilities of these cells to cohere and to undergo normal morphological development. The fibrils thus mimic the ability of intact, wild-type cells to carry out the same rescue. Optimal cohesion rescue by fibrils required calcium and magnesium ions, did not require protein synthesis, but was energy dependent, i.e., sodium azide and sodium cyanide blocked rescue. Cohesion rescue was also blocked by the diazo dye Congo red. Cohesion rescue is genus specific, i.e., isolated fibrils did not cause the cohesion of Pseudomonas aeruginosa, Bacillus subtilis, Proteus mirabilis, Escherichia coli, or the related myxobacterium Stigmatella aurantiaca. Developmental rescue of Dsp by isolated fibrils included aggregation, fruiting body formation, and myxospore morphogenesis. Developmental gene expression in the Dsp mutant was only partially rescued by the isolated fibrils.