Cajal body-specific small nuclear RNAs:: a novel class of 2′-O-methylation and pseudouridylation guide RNAs
Cajal body-specific small nuclear RNAs:: a novel class of 2′-O-methylation and pseudouridylation guide RNAs
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DOI：
10.1093/emboj/21.11.2746
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发表时间：
2002-06-03
期刊：
影响因子：
11.4
通讯作者：
Kiss, T
中科院分区：
文献类型：
--
作者：
Darzacq, X;Jády, BE;Kiss, T
Cajal体是一种存在于动物和植物细胞核质中的保守的亚核细胞器。虽然卡哈尔体在近100年前就被首次描述，但它们的功能在很大程度上仍然是推测性的。在这里，我们描述了一类新的人类小核RNA，专门定位于卡哈尔体。预测或已经证明小Cajal体特异性RNA（scaRNA）在RNA聚合酶II转录的U1、U2、U4和U 5剪接体小核RNA（snRNA）中的2 '-O-核糖甲基化核苷酸和假尿苷的位点特异性合成中起指导RNA的作用。我们的研究结果提供了强有力的支持的想法，卡哈尔体，这个神秘的核细胞器，剪接体snRNAs的转录后修饰提供了细胞场所。
Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their function has remained largely speculative. Here, we describe a novel class of human small nuclear RNAs that localize specifically to Cajal bodies. The small Cajal body- specific RNAs (scaRNAs) are predicted or have already been demonstrated to function as guide RNAs in site-specific synthesis of 2'-O-ribose-methylated nucleotides and pseudouridines in the RNA polymerase II-transcribed U1, U2, U4 and U5 spliceosomal small nuclear RNAs (snRNAs). Our results provide strong support for the idea that the Cajal body, this mysterious nuclear organelle, provides the cellular locale for post-transcriptional modification of spliceosomal snRNAs.