Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates.
Using bead injection to model dispensing of 3-D multicellular spheroids into microtiter plates.
复制标题
使用微珠注射模拟将 3-D 多细胞球体分配到微量滴定板中。
DOI：
10.1016/j.talanta.2017.09.022
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发表时间：
2018
期刊：
影响因子：
6.1
通讯作者：
Spicer,Timothy
中科院分区：
文献类型：
--
作者：
Singhera,Fakhar;Cooper,Emily;Scampavia,Louis;Spicer,Timothy
几十年来，生物医学转化研究一直依赖于二维(2D)细胞培养来进行药物发现，需要细胞在平坦的表面上生长，而平坦的表面并不总是准确地模拟体内的生物状态。三维(3D)细胞培养，也被称为3D球体或有机体，作为细胞组织生长，特别是在模拟癌症肿瘤样结构方面更具生理学意义[1]。虽然目前产生3D球体的方法很有吸引力，但尚未取代用于药物发现工作的2D培养方法，这些方法使用高通量筛选(HTS)，在可扩展性、重复性和兼容性方面存在局限性，主要与传统微滴板的使用有关。提出了一种利用微珠注入的新用途，可重复地将球体和微珠放置到384和1536孔格式的高密度微滴定板中。
Biomedical translational research has relied on two dimensional (2D) cell cultures for drug discovery over the decades, requiring cells to grow on a flat surface which does not always accurately model in vivo biological states. Three dimensional (3D) cell cultures, also known as 3D spheroids or organoids, grow as cellular tissues that are more physiologically relevant especially with respect to emulating cancer tumor-like structures [1]. While attractive, current methods for generating 3D spheroids has yet to replace 2D culturing methods used for drug discovery efforts that employ high-throughput screening (HTS), having limitations with scalability, reproducibility, and compatibility predominantly associated with conventional microtiter plate usage. Presented is a novel use of bead injection for the reproducible placement of spheroids and beads into high density microtiter plates of a 384- and 1536- well format.