Extracellular Matrix Deposition in Engineered Micromass Cartilage Pellet Cultures: Measurements and Modelling.
Extracellular Matrix Deposition in Engineered Micromass Cartilage Pellet Cultures: Measurements and Modelling.
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DOI：
10.1371/journal.pone.0147302
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Please CP
中科院分区：
文献类型：
--
作者：
Lewis MC;MacArthur BD;Tare RS;Oreffo RO;Please CP
本文探讨了工程软骨细胞团中细胞外基质沉积的可能机制。理论研究进行旁边的实验研究测量蛋白多糖和胶原蛋白的体积分数在小鼠软骨细胞（ATDC-5）的细胞团。简单的数学模型，它采用了营养素依赖的蛋白多糖生产率，成功地再现了周边为主的蛋白多糖沉积，实验观察到的增长模式的特点后，培养21天的小球。结果表明，这种不均匀的蛋白聚糖生产是由于营养缺乏的颗粒中心。我们的模型分析进一步表明，蛋白多糖基质的空间均匀分布可以通过用较小尺寸的颗粒启动培养过程来维持。最后，提出了可能的扩展，旨在提高模型预测的早期行为，不同的机制似乎占主导地位的矩阵生产内的颗粒。
This article explores possible mechanisms governing extracellular matrix deposition in engineered cartilaginous cell pellets. A theoretical investigation is carried out alongside an experimental study measuring proteoglycan and collagen volume fractions within murine chondrogenic (ATDC-5) cell pellets. The simple mathematical model, which adopts a nutrient-dependent proteoglycan production rate, successfully reproduces the periphery-dominated proteoglycan deposition, characteristic of the growth pattern observed experimentally within pellets after 21 days of culture. The results suggest that this inhomogeneous proteoglycan production is due to nutrient deficiencies at the pellet centre. Our model analysis further indicates that a spatially uniform distribution of proteoglycan matrix could be maintained by initiating the culture process with a smaller-sized pellet. Finally, possible extensions are put forward with an aim to improve the model predictions for the early behaviour, where different mechanisms appear to dominate the matrix production within the pellets.