Aloe L. -: a second plant family without (TTTAGGG)n telomeres
Aloe L. -: a second plant family without (TTTAGGG)n telomeres
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DOI：
10.1007/s004120050429
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发表时间：
2000-01-01
期刊：
影响因子：
1.6
通讯作者：
Leitch, AR
中科院分区：
文献类型：
--
作者：
Adams, SP;Leitch, IJ;Leitch, AR
染色体的物理末端受到端粒的保护和稳定。端粒DNA序列通常由一个简单的重复单位组成，在许多生物体中是保守的。大多数被检测的植物已经显示具有由序列5 '-TTTAGGG-3'的许多重复拷贝组成的拟南芥型端粒。使用荧光原位杂交，狭缝印迹和不对称聚合酶链反应，我们证明了芦荟属（家庭Asphodelaceae）的arrhopsis型端粒的情况下。迄今为止报道的唯一其他没有这种端粒的植物属是葱属，假蕨属和Tulbaghia（Alliaceae）。由于这些属和芦荟是天门冬目中的花瓣状单子叶植物，因此建议没有拟南芥型端粒可能是这一相关植物组的特征。
The physical ends of chromosomes are protected and stabilised by telomeres. The sequence of telomeric DNA normally consists of a simple repeating unit that is conserved in many organisms. Most plants examined have been shown to possess Arabidopsis-type telomeres consisting of many repeat copies of the sequence 5'-TTTAGGG-3'. Using fluorescent in situ hybridisation, slot blotting and the asymmetric polymerase chain reaction we demonstrate an absence of Arabidopsis-type telomeres in the genus Aloe (family Asphodelaceae). The only other plant genera so far reported without such telomeres are Allium, Nothoscordum, and Tulbaghia (family Alliaceae). As these genera and Aloe are petal-oid monocots in the Asparagales, it is suggested that an absence of Arabidopsis-type telomeres may be characteristic of this related group of plants.