Calculation of hydrodynamic parameters of random coil polymers from size exclusion chromatography and comparison with parameters by conventional methods
Calculation of hydrodynamic parameters of random coil polymers from size exclusion chromatography and comparison with parameters by conventional methods
复制标题
通过尺寸排阻色谱法计算无规卷曲聚合物的流体动力学参数并与常规方法的参数进行比较
DOI：
10.1016/s0021-9673(00)80335-0
复制
发表时间：
1981
影响因子：
4.1
通讯作者：
P. Squire
中科院分区：
文献类型：
--
作者：
P. Squire
一种新的方法处理数据的大小排阻色谱法，最初评估的天然蛋白质，已扩展到无规卷曲聚合物使用的数据从葡聚糖和聚乙二醇作为具体的例子。用已知分子量的球状蛋白校准色谱柱。乘以一个常数，横坐标就变成了分子半径。方程中的常数r = gMZ，然后直接从尺寸排阻色谱数据进行评估，并与从粘度，沉降速度和光散射获得的相应常数进行比较，将斯托克斯半径，均方根端到端距离和回转半径与分子量相关。
A new method of treating data from size exclusion chromatography, originally evaluated for native proteins, has been extended to random coil polymers using data from dextran and polyethylene glycol as specific examples. Columns are calibrated with globular proteins of known molecular weight. Multiplication by a constant transforms the abscissa to molecular radii. The constants in the equationr=gMZare then evaluated directly from the size exclusion chromatographic data and compared with the corresponding constants obtained from viscometry, sedimentation velocity and light scattering, relating Stokes radius, root mean square end-to-end distance and radius of gyration to molecular weight.