Neural progenitor cells grown on hydrogel surfaces respond to the product of the transgene of encapsulated genetically engineered fibroblasts.
Neural progenitor cells grown on hydrogel surfaces respond to the product of the transgene of encapsulated genetically engineered fibroblasts.
复制标题
在水凝胶表面生长的神经祖细胞对封装的基因工程成纤维细胞的转基因产物做出反应。
DOI：
10.1021/bm100699q
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发表时间：
2010
影响因子：
6.2
通讯作者：
Wheatley,MargaretA
中科院分区：
文献类型：
--
作者：
Shanbhag,MihirS;Lathia,JustinD;Mughal,MohamedR;Francis,NicolaL;Pashos,Nicholas;Mattson,MarkP;Wheatley,MargaretA
Engineered tissue strategies for central nervous system (CNS) repair have the potential for localizing treatment using a wide variety of cells or growth factors. However, these strategies are often limited by their ability to address only one aspect of the injury. Here we report the development of a novel alginate construct that acts as a multifunctional tissue scaffold for CNS repair, and as a localized growth factor delivery vehicle. We show that the surface of this alginate construct acts as an optimal growth environment for neural progenitor cell (NPC) attachment, survival, migration, and differentiation. Importantly, we show that tailor-made alginate constructs containing brain-derived neurotrophic factor or neurotrophin-3 differentially direct lineage fates of NPCs and may therefore be useful in treating a wide variety of injuries. It is this potential for directed differentiation of a scaffold prior to implantation at the injury site that we explore here.
