Stimulation of interleukin-6 release by interleukin-1β from isolated human adipocytes
Stimulation of interleukin-6 release by interleukin-1β from isolated human adipocytes
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DOI：
10.1016/s1043-4666(02)00495-7
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发表时间：
2003-01-07
期刊：
影响因子：
3.8
通讯作者：
Mohamed-Ali, V
中科院分区：
文献类型：
--
作者：
Flower, L;Gray, R;Mohamed-Ali, V
白介素6(IL-6)的分泌是由免疫、激素和代谢刺激以细胞特异性的方式调节的。我们研究了细胞因子、肿瘤坏死因子α和IL-1β以及胰岛素对人脂肪细胞和外周血细胞分泌IL-6的影响。脂肪细胞结构性地释放IL-6(5h后：5.64pgml(-1)，10h后：15.95pgml(-1)，p=0.007)，而外周血细胞分泌IL-6无明显变化。5h后，LPS刺激PBC分泌IL-6，但对脂肪细胞无影响。肿瘤坏死因子α和胰岛素可诱导PBC产生IL-6，但对脂肪细胞无影响。然而，IL-1β诱导脂肪细胞和PBC中IL-6的释放显著增加(均P<；0.05)。体内脂肪组织中IL-1β的产生是通过测量腹部皮下脂肪床动静脉的差异来评估的。没有观察到IL-1β的净释放，这表明在基础条件下，这种细胞因子没有从这个库中检测到释放到循环中。结论(1)PBC表现出调节的IL-6释放，而脂肪细胞的释放具有很大的构成成分；(2)免疫调节剂，如内毒素、肿瘤坏死因子和IL-1β，都诱导PBC释放IL-6，但只有IL-1β刺激脂肪细胞的释放。虽然IL-1β不是来自脂肪组织的内分泌信号，但它是人类脂肪细胞释放IL-6的自分泌/旁分泌刺激因子。(C)2003爱思唯尔科学有限公司。保留所有权利。
The secretion of interleukin-6 (IL-6) is modulated by immune, hormonal and metabolic stimuli in a cell-specific manner. We investigated the effect of cytokines, TNFalpha and IL-1beta, and insulin on IL-6 release from human adipocytes and peripheral blood cells (PBC).Adipocytes released IL-6 constitutively (after 5h: 5.64 [1.61-15.30]pg ml(-1), after 10h: 15.95 [2.34-45.59]pg ml(-1), p = 0.007), while PBC secretion did not change significantly over this period. LPS stimulated IL-6 secretion in PBC after 5 h but was without effect on adipocytes. TNFalpha and insulin induced IL-6 production from PBC, but had no effect on adipocytes. IL-1beta, however, induced a substantial increase in IL-6 release in adipocytes and PBC (all p < 0.05).Adipose tissue production of IL-1beta was assessed in vivo by measuring arterio-venous differences across the subcutaneous abdominal adipose bed. Net release of IL-1beta was not observed, suggesting that under basal conditions there is no detectable release of this cytokine into the circulation from this depot.In conclusion (1) PBC demonstrate regulated IL-6 release, while the adipocyte release has a large constitutive component; (2) immune modulators, such as LPS, TNFalpha and IL-1beta, all induce PBC IL-6 release, but only IL-1beta stimulates adipocyte release. Though IL-1beta is not an endocrine signal from adipose tissue, it is an autocrine/paracrine stimulator of IL-6 release from human adipocytes. (C) 2003 Elsevier Science Ltd. All rights reserved.