Functional joint regeneration is achieved using reintegration mechanism in Xenopus laevis.
Functional joint regeneration is achieved using reintegration mechanism in Xenopus laevis.
复制标题
功能关节再生是使用爪诺邦Laevis中的重新整合机制实现的。
DOI：
10.1002/reg2.49
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Agata K
中科院分区：
文献类型：
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作者：
Tsutsumi R;Yamada S;Agata K
一个功能性的关节需要多个组织的整合：相对的骨骼元素应该形成一个互锁的结构，肌肉应该通过横跨关节的肌腱插入骨骼组织。蝾螈在截肢后可以再生功能关节，而非洲爪蟾再生的是没有关节的软骨杆，即“刺”。先前我们报道了蝾螈肘关节再生过程中，剩余组织和再生组织之间的融合机制对再生关节形态发生有重要影响。基于对重新整合的重要性的认识，我们在肘关节处切除了青蛙的四肢，发现青蛙可以在剩余组织和再生的刺突之间再生出一个功能性的肘关节。在再生过程中，再生软骨部分连接到剩余的关节软骨，以改变肘关节近端钉突的联锁结构。此外，剩余部分的肌肉通过肌腱插入再生的刺状软骨。本研究为分析爪蟾关节再生的分子和细胞机制开辟了新的途径。
A functional joint requires integration of multiple tissues: the apposing skeletal elements should form an interlocking structure, and muscles should insert into skeletal tissues via tendons across the joint. Whereas newts can regenerate functional joints after amputation, Xenopus laevis regenerates a cartilaginous rod without joints, a “spike.” Previously we reported that the reintegration mechanism between the remaining and regenerated tissues has a significant effect on regenerating joint morphogenesis during elbow joint regeneration in newt. Based on this insight into the importance of reintegration, we amputated frogs’ limbs at the elbow joint and found that frogs could regenerate a functional elbow joint between the remaining tissues and regenerated spike. During regeneration, the regenerating cartilage was partially connected to the remaining articular cartilage to reform the interlocking structure of the elbow joint at the proximal end of the spike. Furthermore, the muscles of the remaining part inserted into the regenerated spike cartilage via tendons. This study might open up an avenue for analyzing molecular and cellular mechanisms of joint regeneration using Xenopus.