Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium
Proteasome and antioxidant responses in Cottus gobio during a combined exposure to heat stress and cadmium
复制标题
DOI：
10.1016/j.cbpc.2011.10.003
复制
发表时间：
2012-03-01
影响因子：
3.9
通讯作者：
Silvestre, Frederic
中科院分区：
文献类型：
--
作者：
Dorts, Jennifer;Bauwin, Aurelie;Silvestre, Frederic
温度和痕量金属是常见的环境应激源，由于全球气候变化和人为污染，它们的重要性正在增加。研究了镉(名义浓度为0.01和1 mg/L)在15℃和21℃下暴露4天对欧洲牛头鱼肝脏和鳃的氧化损伤和抗氧化特性。首先，暴露于1 mg Cd/L诱导了高死亡率(67%)，在21℃条件下，暴露于0.01 mg Cd/L可显著增加肝脏超氧化物歧化酶活性，降低谷胱甘肽还原酶活性，与热应激无关。在鳃中，与15℃暴露相比，21℃暴露显著提高了鱼的谷胱甘肽过氧化物酶活性，而谷胱甘肽S转移酶活性显著降低。此外，无论Cd胁迫如何，高温处理都显著降低了肝脏中的脂质过氧化水平，显著提高了类糜蛋白酶样20S蛋白酶体的活性。总体而言，目前的结果表明，高温和镉暴露独立地影响了牛头的抗氧化防御系统，具有明显的组织特异性和应激特异性的抗氧化反应。此外，温度升高还会影响肝脏的脂质过氧化和两种组织中20S蛋白酶体的活性。(C)2011 Elsevier Inc.保留所有权利。
Temperature and trace metals are common environmental stressors, and their importance is increasing due to global climate change and anthropogenic pollution. Oxidative damage and antioxidant properties have been studied in liver and gills of the European bullhead (Cottus gobio) subjected to cadmium (CdCl2 at nominal concentrations of 0.01 and 1 mg/L) for 4 days at either 15 degrees C or 21 degrees C First, exposure to 1 mg Cd/L induced a high mortality rate (67%) in fish held at 21 C. Regarding the antioxidant enzymes, exposure to 0.01 mg Cd/L significantly increased the activity of superoxide dismutase (SOD) and decreased the activity of glutathione reductase (GR) in liver, independently of heat stress. In gills, exposure to 21 degrees C resulted in a significantly increased activity of glutathione peroxidase (GPx), whereas the activity of glutathione S-transferase (GST) was significantly reduced as compared to fish exposed to 15 degrees C. Furthermore, regardless of Cd stress, exposure to elevated temperature resulted in a significant decrease of lipid peroxidation (LPO) level in liver and in a significant increase in the activity of chymotrypsin-like 20S proteasome in both studied tissues of C. gobio. Overall, the present results indicated that elevated temperature and cadmium exposure independently influenced the antioxidant defense system in bullhead with clear tissue-specific and stress-specific antioxidant responses. Further, elevated temperature affected the hepatic lipid peroxidation and the activity of 20S proteasome in both tissues. (C) 2011 Elsevier Inc. All rights reserved.