PHYLOGENETIC RELATIONSHIPS OF BRASSICACEAE SPECIES BASED ON MATK SEQUENCES

PHYLOGENETIC RELATIONSHIPS OF BRASSICACEAE SPECIES BASED ON MATK SEQUENCES
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基于matk序列的十字花科物种系统发育关系

DOI:
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发表时间:
2012
影响因子:
1.2
通讯作者:
Wang, Jianbo
Wang, Jianbo
中科院分区:
生物学4区
文献类型:
--
作者:
Liu, Lei;Zhao, Bo;Tan, Dunyan;Wang, Jianbo

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叶绿体基因matK位于叶绿体trnK的内含子中,编码成熟酶,matK的变异为属间水平的系统发育分析提供了实质性的解决方案。利用127种(包括亚种和变种)兰科植物和1个外类群种(醉蝶花)的序列数据,采用邻接法、最大简约法和最大似然法构建了该科的系统发育关系。系统发育的结果普遍证实了最近建立的部落联盟,并指出27个部落中的大多数被分配到血统I-III。我们发现诸葛菜复合体,包括O。violaceus,O. taibaiensis,O. hupehensis和O. diffuses,这是原产于中国,应归入谱系II,并最密切相关的族。南芥属是一个多系分支,其中一个亚分支与Boechebis有姐妹关系;与高山有关的物种形成另一个分支,与任何部落都没有联系。以前的分析将Conringia planisiliqua放在族中,但在目前的分析中,它被包括在Isatideae中,支持以前的假设,它是这个部落的成员。
The chloroplast gene matK, located in the intron of chloroplast trnK, encodes maturase, and variations of matK provide substantial resolution for phylogenetic analyses at intergeneric levels. Sequence data from 127 species (including subspecies and varieties) of Brassicaceae and one outgroup specie (Cleome gynandra) were used to construct the phylogeny of this family and elucidate the phylogenetic relationships therein using the neighbor-joining, maximum parsimony, and maximum likelihood methods. The phylogenetic results generally confirmed recently established tribal alignments and indicated that most of the 27 tribes were assigned to Lineages I–III. We found that the Orychophragmus violaceus complex, including O. violaceus, O. taibaiensis, O. hupehensis, and O. diffuses, which are native to China, should be subsumed under Lineage II, and was most closely associated with the tribe Brassiceae. Arabis was confirmed to be polyphyletic and one subclade shared a sister relationship with Boechereae, while A. alpine related species formed the other clade, which was not associated with any tribes. Previous analyses placed Conringia planisiliqua in tribe Brassiceae, but it was included within Isatideae in the current analyses, supporting previous hypotheses that it was a member of this tribe.
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