Phylogeny and virulence divergency analyses of Toxoplasma gondii isolates from China.
Phylogeny and virulence divergency analyses of Toxoplasma gondii isolates from China.
复制标题
中国弓形虫系统发育及毒力差异分析
DOI：
10.1186/1756-3305-7-133
复制
发表时间：
2014-03-28
影响因子：
3.2
通讯作者：
Shen JL
中科院分区：
文献类型：
--
作者：
Li M;Mo XW;Wang L;Chen H;Luo QL;Wen HQ;Wei W;Zhang AM;Du J;Lu FL;Lun ZR;Shen JL
背景：刚地弓形虫（弓形虫）是一种非常成功的寄生虫，它可以感染几乎所有分布在世界各地的温血动物。它在人类和家畜中引起广泛的临床表现。此外，弓形虫各菌株在致病性和地理分布上存在显著的生物学差异。基于限制性片段长度多态性（RFLP）方法的分子流行病学研究表明，北美和欧洲主要有三种基因型，而世界其他地区则存在其他不同的基因型。微卫星（Microsatellite， MS）作为一种遗传标记在许多生物中得到了广泛的应用。然而，对弓形虫分离物的MS基因分型和指纹图谱的报道有限，对中国主要流行的弓形虫菌株的MS数据知之甚少。方法：利用12条不同染色体上的15个MS标记对28株中国弓形虫分离株进行基因分型。结果用贝叶斯统计方法对种群结构进行了分析。系统发育分析来自Neighbor-Net系统发育网络。通过小鼠接种和细胞侵袭试验对部分代表性分离株进行了毒力分析。采用实时荧光定量PCR （qRT- PCR）检测部分毒力相关因子（VFs）的基因表达。结果：28株分离株均聚集在3个单倍群中，但单倍群间存在较小的遗传差异。大多数菌株属于一个单倍群，与先前描述的中国1型（toxodb# 9）相对应。系统发育网络揭示了弓形虫菌株的有限多样性，并且具有相同基因型的菌株的毒力不同。然而，除了致密颗粒蛋白3 （GRA3）在低毒力菌株TgCtwh6 （Wh6）中的表达量高于高毒力菌株TgCtwh3 （Wh3）外，各组间VFs无显著差异。结论：尽管中国1型弓形虫毒力存在差异，但中国1型弓形虫微卫星分型数据显示其遗传多样性有限，所选VFs和系统发育网络分析显示差异较小。
Background:Toxoplasma gondii (T. gondii) is a very successful parasite that can infect virtually all warm blooded animals with a worldwide distribution. It causes a large range of clinical manifestations in both humans and domesticated animals. In addition, marked biological differences exist among T. gondii strains in the pathogenicity and geographical distribution. Molecular epidemiology studies primarily based on restriction fragment length polymorphism (RFLP) method revealed that three main types are predominant in North America and Europe, whereas other diverse genotypes are found in other parts of the world. Microsatellite (MS) as a type of genetic marker has been widely used in many organisms. Limited MS genotyping, however, to fingerprint T. gondii isolates has been reported and little is known about the MS data of the strains predominantly prevalent in China.Methods:Genotyping of twenty-eight Chinese T. gondii isolates were performed using 15 MS markers located on 12 different chromosomes. Results were analyzed in terms of population structure by a Bayesian statistical approach. Phylogenetic analysis was obtained from a Neighbor-Net phylogenetic network. The virulence analyses of some representative isolates were determined by inoculation of mice and cell invasion assays. The gene expressions of some virulence-associated factors (VFs) were performed by quantitative real-time PCR (qRT- PCR).Results:Three haplogroups were clustered among the 28 isolates although minor genetic differences were found within haplogroups. The majority of strains belong to one haplogroup corresponding to the previously described Chinese 1 type (ToxoDB#9). Phylogenetic networks uncovered a limited diversity of T. gondii strains and the virulence differs in the strains sharing the same genotype. No remarkable difference, however, was noted in the tested VFs except for dense granule protein3 (GRA3), which was found to have a higher expression in low virulent TgCtwh6 (Wh6) strain than that in high virulent TgCtwh3 (Wh3) strain.Conclusion:The profile of microsatellite typing data from Chinese T. gondii strains revealed a limited genetic diversity and the selected VFs and phylogenetic network analyses displayed less divergence, although the strain virulence differs in the Chinese 1 type of T. gondii predominantly prevalent in China.
登录
查看更多内容
影响因子：
--
作者：
Kim, Ji Yeon;Ahn, Hye-Jin;Nam, Ho-Woo
通讯作者：
Nam, Ho-Woo
影响因子：
5.4
作者：
Feng, PH;Park, J;Jung, JU
通讯作者：
Jung, JU
影响因子：
4
作者：
Dubey, J. P.;Velmurugan, G. V.;Su, C.
通讯作者：
Su, C.
影响因子：
3.1
作者：
Howe, DK;Summers, BC;Sibley, LD
通讯作者：
Sibley, LD
影响因子：
6.4
作者：
Ajzenberg, Daniel;Yera, Helene;Villena, Isabelle
通讯作者：
Villena, Isabelle