Ehrlichia minasensis sp nov., isolated from the tick Rhipicephalus microplus
Ehrlichia minasensis sp nov., isolated from the tick Rhipicephalus microplus
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DOI：
10.1099/ijsem.0.000895
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发表时间：
2016-03-01
影响因子：
2.8
通讯作者：
de la Fuente, Jose
中科院分区：
文献类型：
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作者：
Cabezas-Cruz, Alejandro;Zweygarth, Erich;de la Fuente, Jose
最近，我们获得了立克次体分离物（埃立克体属。UFMG-EVT）的血淋巴充血的微小扇头蜱雌性。利用16 S rRNA基因、groEL、dsb、gltA和trp 36序列进行系统发育分析，结果表明，该菌属于1-变形菌门无形体科埃里希体属。Ehrlichia sp. UFMG-EVT是犬埃里希体的姐妹分类群，其16 S rRNA基因、groEL、dsb、gltA和trp 36序列的相似性分别为98.3%、97.25、94.7%、94.3%和49.15。在实验室中，通过在IDE 8蜱细胞系中连续传代维持埃立克体属UFMG-EVT，其中使用电子显微镜表征超微结构，发现其与埃立克体相似。犬埃立克体、鼠埃立克体和查菲埃立克体，但未检测到反刍埃立克体和伊文氏埃立克体。我们建议将这种细菌命名为米纳斯埃里希体新种，以确认它最初分离的地方，巴西的米纳斯吉拉斯。模式菌株为埃里希体属菌株UFMG-EVT（=DSM 100393（T）=TCB-TBB-0018（T））。
Recently, we obtained a rickettsial isolate (Ehrlichia sp. UFMG-EVT) from the haemolymph of engorged Rhipicephalus microplus tick females. On the basis of maximum-likelihood phylogenetic analysis using 16S rRNA gene, groEL, dsb, gltA and trp36 sequences we showed that Ehrlichia sp. UFMG-EVT belongs to the 1-Proteobacteria, family Anaplasmataceae, genus Ehrlichia. Ehrlichia sp. UFMG-EVT is a sister taxon of Ehrlichia canis with 16S rRNA gene, groEL, dsb, gltA and trp36 sequence similarities of 98.3 %, 97.2 5, 94.7 %, 94.3 % and 49.1 5, respectively. Ehrlichia sp. UFMG-EVT has been maintained in the laboratory by continuous passage in the IDE8 tick cell line where the ultrastructure was characterized using electron microscopy and was found to resemble that of E. canis, Ehrlichia muris and Ehrlichia chaffeensis, but not Ehrlichia ruminantium and Ehrlichia ewingii. We propose the name Ehrlichia minasensis sp. nov. for this bacterium to acknowledge the place from where it was initially isolated, Minas Gerais, Brazil. The type strain is strain Ehrlichia sp. UFMG-EVT (=DSM 100393(T)=TCB-TBB-0018(T)).